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IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibody at dilution of 1:100 (40x lens).)
Mouse anti-Human, Rat PARP Polyclonal Antibody | anti-PARP antibody
Mouse monoclonal to Human PARP
Reactivity
Human, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
PARP, Antibody; Mouse monoclonal to Human PARP; PARP Mouse Mab Antibody; anti-PARP antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
Liquid
Applicable Applications for anti-PARP antibody
WB (Western Blot)
Immunogen
Synthetic peptide of human PARP, conjugated to KLH.
Storage Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Related Product Information for anti-PARP antibody
PARP, a 116 kDa nuclear poly (ADPribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICElike caspases in vitro (2,3) and is one of the main cleavage targets of caspase3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP aminoterminal DNA binding domain (24 kDa) from the carboxyterminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
References
1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356358.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346347.
3. Cohen, G.M. (1997) Biochem. J. 326, 116.
4. Nicholson, D. W. et al. (1995) Nature 376, 3743.
5. Tewari, M. et al. (1995) Cell 81, 801809.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346347.
3. Cohen, G.M. (1997) Biochem. J. 326, 116.
4. Nicholson, D. W. et al. (1995) Nature 376, 3743.
5. Tewari, M. et al. (1995) Cell 81, 801809.
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Product Notes
The PARP (Catalog #AAA36780) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal to Human PARP reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PARP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PARP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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