Goat PAX3 Polyclonal Antibody | anti-PAX3 antibody

Goat anti-PAX3 Antibody

Cat. Num. AAA60917

• Gene Names: PAX3; WS1; WS3; CDHS; HUP2
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PAX3, Antibody; Goat anti-PAX3 Antibody; PAX3; WS1; CDHS; HUP2; paired box gene 3 (Waardenburg syndrome 1); paired domain gene 3; paired box homeotic gene 3 (Waardenburg syndrome 1); paired box 3; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; paired domain ge; PAX3 antibody; WS1 antibody; CDHS antibody; HUP2 antibody; paired box gene 3 (Waardenburg syndrome 1) antibody; paired domain gene 3 antibody; paired box homeotic gene 3 (Waardenburg syndrome 1) antibody; paired box 3 antibody; MGC120381 antibody; MGC120382 antibody; MGC120383 antibody; MGC120384 antibody; MGC134778 antibody; paired box homeotic gene 3 antibody; paired domain gene HuP2 antibody; anti-PAX3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-PAX3 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_000429.2; NP_039230.1; NP_852122.1;.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1.
• Epitope: N Terminus
• Note: This antibody is expected to recognise all 8 reported isoforms (as represented by NP_000429.2; NP_039230.1; NP_852122.1;.1 NP_852123.1; NP_852124.1; NP_852125.1 and NP_852126.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

IHC (Immunohistochemisry)

(AAA60917 Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.)

IHC (Immunohiostchemistry)

(AAA60917 (4.5ug/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

WB (Western Blot)

(AAA60917 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

References

Hsieh MJ, Yao YL, Lai IL, Yang WM.; Transcriptional repression activity of PAX3 is modulated by competition between corepressor KAP1 and heterochromatin protein 1.; Biochem Biophys Res Commun.2006 Oct 20;349(2): 573-81.; Pubmed 16945326

NCBI and Uniprot Product Information

• NCBI GI #: 6654638
• NCBI GeneID: 5077
• NCBI Accession #: NP_000429.2; NP_039230.1; NP_852122.1; .1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1
• NCBI GenBank Nucleotide #: NM_000438.5
• NCBI Official Full Name: paired box protein Pax-3 isoform PAX3a
• NCBI Official Synonym Full Names: paired box 3
• NCBI Official Symbol: PAX3
• NCBI Official Synonym Symbols: WS1; WS3; CDHS; HUP2
• NCBI Protein Information: paired box protein Pax-3
• UniProt Protein Name: Paired box protein Pax-3
• UniProt Gene Name: PAX3
• UniProt Synonym Gene Names: HUP2
• UniProt Entry Name: PAX3_HUMAN