FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PAX3 Polyclonal Antibody | anti-PAX3 antibody
PAX3 Antibody (N-term)
Gene Names
PAX3; WS1; WS3; CDHS; HUP2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PAX3, Antibody; PAX3 Antibody (N-term); Paired box protein Pax-3; HuP2; PAX3; HUP2; anti-PAX3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PAX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the N-terminal region of human PAX3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
98-126
Sequence Length
505
Applicable Applications for anti-PAX3 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(PAX3 Antibody (N-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the PAX3 antibody detected the PAX3 protein (arrow).)
WB (Western Blot)
(PAX3 Antibody (N-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the PAX3 antibody detected the PAX3 protein (arrow).)
Related Product Information for anti-PAX3 antibody
PAX3 probable is a transcription factor associated with development of alveolar rhabdomyosarcoma.
Product Categories/Family for anti-PAX3 antibody
References
Boutet,S.C., et.al., Cell 130 (2), 349-362 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52968
NCBI Official Full Name
paired box protein Pax-3 isoform PAX3a
NCBI Official Synonym Full Names
paired box 3
NCBI Official Symbol
PAX3
NCBI Official Synonym Symbols
WS1; WS3; CDHS; HUP2
NCBI Protein Information
paired box protein Pax-3
UniProt Protein Name
Paired box protein Pax-3
UniProt Gene Name
PAX3
UniProt Synonym Gene Names
HUP2
UniProt Entry Name
PAX3_HUMAN
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Product Notes
The PAX3 pax3 (Catalog #AAA287241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 98-126. The PAX3 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PAX3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PAX3 pax3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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