IHC (Immunohistochemisry)
(PAX5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit PAX5 Polyclonal Antibody | anti-PAX5 antibody
PAX5 Antibody (Center)
Gene Names
PAX5; ALL3; BSAP
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PAX5, Antibody; PAX5 Antibody (Center); Paired box protein Pax-5; B-cell-specific transcription factor; BSAP; PAX5; anti-PAX5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PAX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the Central region of human PAX5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.3 (varies by lot)
Sequence Positions
178-207
Sequence Length
357
Applicable Applications for anti-PAX5 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(PAX5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(PAX5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(PAX5 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PAX5 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(PAX5 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PAX5 antibody detected the PAX5 protein (arrow).)
WB (Western Blot)
(PAX5 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PAX5 antibody detected the PAX5 protein (arrow).)
Related Product Information for anti-PAX5 antibody
This gene encodes a member of the paired box (PAX) family
of transcription factors. The central feature of this gene family
is a novel, highly conserved DNA-binding motif, known as the paired
box. PAX proteins are important regulators in early development,
and alterations in the expression of their genes are thought to
contribute to neoplastic transformation. This gene encodes the
B-cell lineage specific activator protein that is expressed at
early, but not late stages of B-cell differentiation. Its
expression has also been detected in developing CNS and testis and
so the encoded protein may also play a role in neural development
and spermatogenesis. This gene is located at 9p13, which is
involved in t(9;14)(p13;q32) translocations recurring in small
lymphocytic lymphomas of the plasmacytoid subtype, and in derived
large-cell lymphomas. This translocation brings the potent E-mu
enhancer of the IgH gene into close proximity of the PAX5 promoter,
suggesting that the deregulation of transcription of this gene
contributes to the pathogenesis of these lymphomas. Alternatively
spliced transcript variants encoding different isoforms have been
described but their biological validity has not been determined.
of transcription factors. The central feature of this gene family
is a novel, highly conserved DNA-binding motif, known as the paired
box. PAX proteins are important regulators in early development,
and alterations in the expression of their genes are thought to
contribute to neoplastic transformation. This gene encodes the
B-cell lineage specific activator protein that is expressed at
early, but not late stages of B-cell differentiation. Its
expression has also been detected in developing CNS and testis and
so the encoded protein may also play a role in neural development
and spermatogenesis. This gene is located at 9p13, which is
involved in t(9;14)(p13;q32) translocations recurring in small
lymphocytic lymphomas of the plasmacytoid subtype, and in derived
large-cell lymphomas. This translocation brings the potent E-mu
enhancer of the IgH gene into close proximity of the PAX5 promoter,
suggesting that the deregulation of transcription of this gene
contributes to the pathogenesis of these lymphomas. Alternatively
spliced transcript variants encoding different isoforms have been
described but their biological validity has not been determined.
Product Categories/Family for anti-PAX5 antibody
References
Song, J., et al. Arch. Pathol. Lab. Med. 134(11):1702-1705(2010)
Okamoto, R., et al. Haematologica 95(9):1481-1488(2010)
Best, A., et al. Leuk. Res. 34(8):1098-1102(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Vidal, L.J., et al. Mol. Cancer Res. 8(3):444-456(2010)
Okamoto, R., et al. Haematologica 95(9):1481-1488(2010)
Best, A., et al. Leuk. Res. 34(8):1098-1102(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Vidal, L.J., et al. Mol. Cancer Res. 8(3):444-456(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42149
NCBI Official Full Name
paired box protein Pax-5 isoform 2
NCBI Official Synonym Full Names
paired box 5
NCBI Official Symbol
PAX5
NCBI Official Synonym Symbols
ALL3; BSAP
NCBI Protein Information
paired box protein Pax-5
UniProt Protein Name
Paired box protein Pax-5
UniProt Gene Name
PAX5
UniProt Synonym Gene Names
BSAP
UniProt Entry Name
PAX5_HUMAN
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Product Notes
The PAX5 pax5 (Catalog #AAA285925) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 178-207. The PAX5 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PAX5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PAX5 pax5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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