IHC (Immunohiostchemistry)
(AAA327219 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit PAX6 Polyclonal Antibody | anti-PAX6 antibody
PAX6 Antibody
Gene Names
PAX6; AN; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PAX6, Antibody; PAX6 Antibody; AN 2; AN; AN2; Aniridia type II protein; D11S812E; FVH1; KIAA0552; Leucine zipper putative tumor suppressor 3; LZTS3; MGC17209; MGDA; Oculorhombin; Paired box 6; Paired box gene 6 (aniridia keratitis); Paired Box Gene 6; Paired box homeotic gene 6; Paired box protein Pax-6; Paired box protein Pax6; PAX 6; PAX6; PAX6_HUMAN; ProSAP-interacting protein 1; PROSAPIP1; Sey; WAGR; anti-PAX6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PAX6 antibody detects endogenous levels of total PAX6
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
422
Applicable Applications for anti-PAX6 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PAX6
Subcellular Location
Nucleus.
Tissue Specificity
Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327219 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327219 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of PAX6 using K562 whole cell lysates)
WB (Western Blot)
(Western blot analysis of PAX6 using K562 whole cell lysates)
Related Product Information for anti-PAX6 antibody
Function: Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
Subunit Structure: Interacts with MAF and MAFB. Interacts with TRIM11; this interaction leads to ubiquitination and proteasomal degradation, as well as inhibition of transactivation, possibly in part by preventing PAX6 binding to consensus DNA sequences.
Post-translational Modifications: Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
Similarity: Belongs to the paired homeobox family.
Subunit Structure: Interacts with MAF and MAFB. Interacts with TRIM11; this interaction leads to ubiquitination and proteasomal degradation, as well as inhibition of transactivation, possibly in part by preventing PAX6 binding to consensus DNA sequences.
Post-translational Modifications: Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
Similarity: Belongs to the paired homeobox family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 47 kDa
Predicted: 47 kDa
Predicted: 47 kDa
NCBI Official Full Name
paired box protein Pax-6 isoform a
NCBI Official Synonym Full Names
paired box 6
NCBI Official Symbol
PAX6
NCBI Official Synonym Symbols
AN; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
NCBI Protein Information
paired box protein Pax-6
UniProt Protein Name
Paired box protein Pax-6
UniProt Gene Name
PAX6
UniProt Synonym Gene Names
AN2
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Product Notes
The PAX6 pax6 (Catalog #AAA327219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAX6 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAX6 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PAX6 pax6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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