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product-image-AAA211798_WB13.jpg WB (Western Blot) (Detection of human PBK by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PBK antibody AAA211798 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PBK was successfully immunoprecipitated by rabbit anti-PBK antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)

Rabbit anti-Human PBK Polyclonal Antibody | anti-PBK antibody

Rabbit anti-PBK Antibody, Affinity Purified

Gene Names
PBK; SPK; CT84; TOPK; Nori-3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PBK, Antibody; Rabbit anti-PBK Antibody, Affinity Purified; HEL164; cancer/testis antigen 84; SPK; CT84; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase; serine/threonine protein kinase; PDZ-binding kinase; Nori-3; MAPKK-like protein kinase; lymphokine-activated killer T-cell-originated protein kinase; epididymis luminal protein 164; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; PDZ binding kinase; anti-PBK antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PBK antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human PBK by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PBK antibody AAA211798 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PBK was successfully immunoprecipitated by rabbit anti-PBK antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)

product-image-AAA211798_WB13.jpg WB (Western Blot) (Detection of human PBK by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PBK antibody AAA211798 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PBK was successfully immunoprecipitated by rabbit anti-PBK antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)

IHC (Immunohistochemistry)

(Detection of human PBK by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PBK (Cat. No. AAA211798 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA211798_IHC15.jpg IHC (Immunohistochemistry) (Detection of human PBK by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PBK (Cat. No. AAA211798 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-PBK antibody
PBK (PDZ binding kinase), also known as TOPK (T-LAK cell-originated protein), is a mitogen-activated protein kinase kinase (MAPKK) first identified in a yeast two-hybrid assay as a Dlg1 (disks-large)-interacting protein. PBK has been found to be up-regulated in breast cancers and highly expressed in neural stem cell cultures.
Product Categories/Family for anti-PBK antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,085 Da
NCBI Official Full Name
lymphokine-activated killer T-cell-originated protein kinase
NCBI Official Synonym Full Names
PDZ binding kinase
NCBI Official Symbol
PBK
NCBI Official Synonym Symbols
SPK; CT84; TOPK; Nori-3
NCBI Protein Information
lymphokine-activated killer T-cell-originated protein kinase; PDZ-binding kinase; cancer/testis antigen 84; MAPKK-like protein kinase; serine/threonine protein kinase; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase
UniProt Protein Name
Lymphokine-activated killer T-cell-originated protein kinase
UniProt Gene Name
PBK
UniProt Synonym Gene Names
TOPK; CT84; SPK
UniProt Entry Name
TOPK_HUMAN

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Product Notes

The PBK pbk (Catalog #AAA211798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PBK Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PBK can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PBK pbk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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