WB (Western Blot)
(Detection of human and mouse PCAF by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCAF antibody AAA211581 (lot AAA211581-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PCAF Polyclonal Antibody | anti-PCAF antibody
Rabbit anti-PCAF Antibody, Affinity Purified
Gene Names
KAT2B; P; CAF; PCAF; P/CAF
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PCAF, Antibody; Rabbit anti-PCAF Antibody, Affinity Purified; K(lysine) acetyltransferase 2B; p300/CBP-associated factor; PCAF; P/CAF; Lysine acetyltransferase 2B; histone acetyltransferase KAT2B; histone acetylase PCAF; CAF; histone acetyltransferase PCAF; CREBBP-associated factor; Histone acetyltransferase KAT2B; lysine acetyltransferase 2B; anti-PCAF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PCAF antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 782 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PCAF by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCAF antibody AAA211581 (lot AAA211581-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse PCAF by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCAF antibody AAA211581 (lot AAA211581-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PCAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCAF antibody AAA211581 (lot AAA211581-2) used for IP at 6 ug per reaction. PCAF was also immunoprecipitated by a previous lot of this antibody (lot AAA211581-1). For blotting immunoprecipitated PCAF, AAA211581 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PCAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCAF antibody AAA211581 (lot AAA211581-2) used for IP at 6 ug per reaction. PCAF was also immunoprecipitated by a previous lot of this antibody (lot AAA211581-1). For blotting immunoprecipitated PCAF, AAA211581 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
ChIP (Chromatin Immunoprecipitation)
(Localization of PCAF Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-PCAF antibody AAA211581 and analyzed by DNA sequencing. The figure illustrates the peak distribution of PCAF binding within a 650 Kb region of chromosome 1 as detected using anti-PCAF antibody AAA211581. ChIP-seq validation performed by Diogenode, Denville, NJ.)
ChIP (Chromatin Immunoprecipitation)
(Localization of PCAF Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-PCAF antibody AAA211581 and analyzed by DNA sequencing. The figure illustrates the peak distribution of PCAF binding within a 650 Kb region of chromosome 1 as detected using anti-PCAF antibody AAA211581. ChIP-seq validation performed by Diogenode, Denville, NJ.)
Related Product Information for anti-PCAF antibody
PCAF (P300/CBP-associated factor) is a histone acetyltransferase (HAT) identified as a factor that associates with P300/CBP, a transcriptional coactivator of nuclear hormone receptors and other transcription factors.
Product Categories/Family for anti-PCAF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,013 Da
NCBI Official Full Name
histone acetyltransferase KAT2B
NCBI Official Synonym Full Names
K(lysine) acetyltransferase 2B
NCBI Official Symbol
KAT2B
NCBI Official Synonym Symbols
P; CAF; PCAF; P/CAF
NCBI Protein Information
histone acetyltransferase KAT2B; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF
UniProt Protein Name
Histone acetyltransferase KAT2B
UniProt Gene Name
KAT2B
UniProt Synonym Gene Names
PCAF; Histone acetylase PCAF; P/CAF
UniProt Entry Name
KAT2B_HUMAN
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Product Notes
The PCAF kat2b (Catalog #AAA211581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PCAF Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCAF can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PCAF kat2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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