IHC (Immunohiostchemistry)
(IHC analyses of human HCC samples were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)
Rabbit anti-Human PCK1 Polyclonal Antibody | anti-PCK1 antibody
PCK1 (Phospho-Ser90) Antibody
Gene Names
PCK1; PEPCK1; PEPCKC; PEPCK-C
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purifiedfrom rabbitantiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody againstnon-phosphopeptidewas removed by chromatographyusing non-phosphopeptidecorresponding tothephosphorylationsite.
Synonyms
PCK1, Antibody; PCK1 (Phospho-Ser90) Antibody; anti-PCK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PCK1 (Phospho-Ser90) antibody detects endogenous levels of PCK1 only when phosphorylated at serine90.
Purity/Purification
The antibody was affinity-purifiedfrom rabbitantiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody againstnon-phosphopeptidewas removed by chromatographyusing non-phosphopeptidecorresponding tothephosphorylationsite.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl,0.02% sodium azide.
Applicable Applications for anti-PCK1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from Human PCK1 around the phosphorylation site of serine 90.
Preparation and Storage
Store at -20 degree C for 1 year
IHC (Immunohiostchemistry)
(IHC analyses of human HCC samples were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)
IHC (Immunohiostchemistry)
(IHC analyses of human HCC samples were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)
Application Data
(Huh7 cells expressing DYKDDDDK-PCK1 were treated with or without IGF1 (100 ng/ml) for 1 h. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)
Application Data
(Huh7 cells expressing DYKDDDDK-PCK1 were treated with or without IGF1 (100 ng/ml) for 1 h. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies in the presence or absence of a PCK1 pS90 blocking peptide.)
Related Product Information for anti-PCK1 antibody
Phosphoenolpyruvate carboxykinase (PCK) is the rate-limiting enzyme of gluconeogenesis in the liver and kidney and converts oxaloacetate and GTP into phosphoenolpyruvate (PEP) and CO2 by adding a phosphate to pyruvate with concomitant aldol cleavage of CO2 from oxaloacetate 1,2. In humans, cytosolic PCK1 shares 63.4% sequence identity with PCK2, which is located in the mitochondria 3. Aberrant PCK expression occurs in many cancers. For instance, PCK1 is overexpressed in melanoma and colorectal cancer, and PCK2 is highly expressed in breast, colon and lung cancer cells4-8. In a recent study, it is reported that AKT in tumor cells phosphorylates cytosolic phosphoenolpyruvate carboxykinase 1 (PCK1) at S909. Phosphorylated PCK1 translocates to the ER, where PCK1 uses GTP as a phosphate donor to phosphorylate Insig1 S207 and Insig2 S151. This phosphorylation reduces the binding of sterol to Insig1/2 and disrupts Insig-SCAP interaction, leading to SCAP/SREBP1 translocation to the Golgi apparatus and subsequent SREBP1 activation and downstream gene transcription for lipogenesis, tumor cell proliferation, and tumorigenesis in mice9.
Product Categories/Family for anti-PCK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
622
NCBI Official Full Name
Phosphoenolpyruvate carboxykinase, cytosolic
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 1 (soluble)
NCBI Official Symbol
PCK1
NCBI Official Synonym Symbols
PEPCK1; PEPCKC; PEPCK-C
NCBI Protein Information
phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase
UniProt Protein Name
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
UniProt Gene Name
PCK1
UniProt Synonym Gene Names
PEPCK1; PEPCK-C
UniProt Entry Name
PCKGC_HUMAN
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Product Notes
The PCK1 pck1 (Catalog #AAA312985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCK1 (Phospho-Ser90) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PCK1 pck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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