WB (Western Blot)
(Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse PCM1 Polyclonal Antibody | anti-PCM1 antibody
Rabbit anti-PCM1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PCM1, Antibody; Rabbit anti-PCM1 Antibody, Affinity Purified; PCM-1; PTC4; RET/PCM-1; hPCM-1; pericentriolar material 1, PCM1; pericentriolar material 1 protein; Pericentriolar material 1 protein; pericentriolar material 1; anti-PCM1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PCM1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1925 and 1975
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PCM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for IP at 6 ug per reaction. PCM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211335-1) and rabbit anti-PCM1 antibody For blotting immunoprecipitated PCM1, AAA211335 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PCM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCM1 antibody AAA211335 (lot AAA211335-2) used for IP at 6 ug per reaction. PCM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211335-1) and rabbit anti-PCM1 antibody For blotting immunoprecipitated PCM1, AAA211335 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PCM1 antibody
PCM1 (pericentriolar material protein 1) is involved in centrosomal trafficking and may function to transport centrosomal proteins to the centrosome along microtubules. Knockdown of PCM1 results in defects in centrosomal assembly, cell cycle arrest in G0 or G1, and activation of the p38-dependent stress pathway and the p53-dependent cell cycle checkpoint. PCM1 is implicated in the susceptibility to schizophrenia.
Product Categories/Family for anti-PCM1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PCM1 pcm1 (Catalog #AAA211335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PCM1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PCM1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PCM1 pcm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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