WB (Western Blot)
(Dilution: Sample:Lane 1: HT1080 (Human) Cell Lysate at 30 ugLane 2: SH-SY5Y (Human) Cell Lysate at 30 ugPrimary: Anti-PDE5A (bs-2349R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 110 kD)
Rabbit PDE5A Polyclonal Antibody | anti-PDE5A antibody
PDE5A Polyclonal Antibody
Gene Names
PDE5A; CN5A; PDE5; CGB-PDE
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PDE5A, Antibody; PDE5A Polyclonal Antibody; cGMP-specific 3'; 5'-cyclic phosphodiesterase; 3.1.4.35; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE; PDE5A; PDE5; anti-PDE5A antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-PDE5A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Dilution: Sample:Lane 1: HT1080 (Human) Cell Lysate at 30 ugLane 2: SH-SY5Y (Human) Cell Lysate at 30 ugPrimary: Anti-PDE5A (bs-2349R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 110 kD)
WB (Western Blot)
(Dilution: Sample:Lane 1: HT1080 (Human) Cell Lysate at 30 ugLane 2: SH-SY5Y (Human) Cell Lysate at 30 ugPrimary: Anti-PDE5A (bs-2349R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 110 kD)
FCM/FACS (Flow Cytometry)
(Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2 ug in 100 uL1X PBS containing 0.5% BSA(green).)
FCM/FACS (Flow Cytometry)
(Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2 ug in 100 uL1X PBS containing 0.5% BSA(green).)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (PDE5A) Polyclonal Antibody, Unconjugated (bs-2349R) at 1400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (PDE5A) Polyclonal Antibody, Unconjugated (bs-2349R) at 1400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
NCBI Official Synonym Full Names
phosphodiesterase 5A
NCBI Official Symbol
PDE5A
NCBI Official Synonym Symbols
CN5A; PDE5; CGB-PDE
NCBI Protein Information
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Protein Name
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Gene Name
PDE5A
UniProt Synonym Gene Names
PDE5; CGB-PDE
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Product Notes
The PDE5A pde5a (Catalog #AAA291940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE5A Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PDE5A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDE5A pde5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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