WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Hep G2; B:MOLT4) using a 12% SDS PAGE gel and PDHA1 antibody at a dilution of 1:1000)
Rabbit PDHA1 Polyclonal Antibody | anti-PDHA1 antibody
PDHA1 Antibody
Gene Names
PDHA1; PDHA; PDHAD; PHE1A; PDHCE1A
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
PDHA1, Antibody; PDHA1 Antibody; Affinity Purified Rabbit Polyclonal PDHA1 Antibody; Polyclonal PDHA1 antibody; Anti-PDHA1 antibody; PDHE1-A type I antibody; PDHA-1; pyruvate dehydrogenase complex E1-alpha polypeptide 1 antibody; PDHA 1; PDHA-1 antibody; PDHA antibody; pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial antibody; PDHA1; PHE1A antibody; PDHCE1A antibody; Pyruvate Dehydrogenase E1 alpha antibody; PDHA 1 antibody; anti-PDHA1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
390
Applicable Applications for anti-PDHA1 antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
PDHA1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 39 and 325
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Hep G2; B:MOLT4) using a 12% SDS PAGE gel and PDHA1 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Hep G2; B:MOLT4) using a 12% SDS PAGE gel and PDHA1 antibody at a dilution of 1:1000)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha antibody at 1:200 dilution.)
WB (Western Blot)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 10% SDS PAGE gel and PDHA1 antibody at a dilution of 1:5000)
WB (Western Blot)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 10% SDS PAGE gel and PDHA1 antibody at a dilution of 1:5000)
Related Product Information for anti-PDHA1 antibody
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 dihydrolipoyl transacetylase; and dihydrolipoyl dehydrogenase. The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits.
Product Categories/Family for anti-PDHA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43 kDa
NCBI Official Full Name
pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 1
NCBI Official Synonym Full Names
pyruvate dehydrogenase E1 alpha 1 subunit
NCBI Official Symbol
PDHA1
NCBI Official Synonym Symbols
PDHA; PDHAD; PHE1A; PDHCE1A
NCBI Protein Information
pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
UniProt Protein Name
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
UniProt Gene Name
PDHA1
UniProt Synonym Gene Names
PHE1A
UniProt Entry Name
ODPA_HUMAN
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Product Notes
The PDHA1 pdha1 (Catalog #AAA225202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDHA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PDHA1 pdha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDHA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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