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IP (Immunoprecipitation)
(PDHX was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-PDHX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDHX rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)
Rabbit anti-Human PDHX Polyclonal Antibody | anti-PDHX antibody
Anti-PDHX Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PDHX, Antibody; Anti-PDHX Antibody, Rabbit Polyclonal; PDHX Antibody, Rabbit PAb, Antigen Affinity Purified; pyruvate dehydrogenase complex, component X; Anti-DLDBP Antibody; Anti-E3BP Antibody; Anti-OPDX Antibody; Anti-PDX1 Antibody; Anti-proX Antibody; anti-PDHX antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PDHX
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PDHX antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human PDHX fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PDHX fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PDHX was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-PDHX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDHX rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)
IP (Immunoprecipitation)
(PDHX was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-PDHX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDHX rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of PDHX in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDHX polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of PDHX in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDHX polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PDHX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PDHX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PDHX rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: NCI H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDa)
WB (Western Blot)
(Anti-PDHX rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: NCI H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDa)
Related Product Information for anti-PDHX antibody
PDHX (Pyruvate Dehydrogenase Complex Component X) is a Protein Coding gene. The PDHX gene, located on 11p13, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, chicken, zebrafish, and frog. This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). Among its related pathways are Pyruvate metabolism and Citric Acid (TCA) cycle and Signaling by GPCR.
Product Categories/Family for anti-PDHX antibody
References
Ivanov IS, et al. (2014) Founder p.Arg 446* mutation in the pdhx gene explains over half of cases with congenital lactic acidosis in roma children. Mol Genet Metab 113 (1-2): 76-83.Brown RM, et al. (2006) Pyruvate dehydrogenase e3 binding protein (protein x) deficiency. Dev Med Child Neurol 48 (9): 756-760.Bottone MG, et al. (2007) Enzyme-assisted photosensitization with rose bengal acetate induces structural and functional alteration of mitochondria in hela cells. Histochem Cell Biol 127 (3): 263-271.
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Product Notes
The PDHX (Catalog #AAA256631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDHX Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDHX can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDHX for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDHX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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