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IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed A431, using PDI antibody at 1:200 dilution.)
Rabbit PDI Polyclonal Antibody | anti-PDI antibody
PDI Antibody
Gene Names
P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
PDI, Antibody; PDI Antibody; Affinity Purified Rabbit Polyclonal PDI Antibody; Polyclonal PDI antibody; Anti-PDI antibody; protein disulfide isomerase family A member 1 antibody; PDI antibody; protein disulfide isomerase/oxidoreductase antibody; P4HB antibody; P4Hbeta antibody; GIT antibody; PDIA1 antibody; glutathione-insulin transhydrogenase antibody; DSI antibody; cellular thyroid hormone-binding protein antibody; protein disulfide-isomerase antibody; ERBA2L antibody; PHDB antibody; PO4HB antibody; PO4DB antibody; PROHB antibody; anti-PDI antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
24
Applicable Applications for anti-PDI antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
PDI antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 23 and 227 of PDI
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed A431, using PDI antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed A431, using PDI antibody at 1:200 dilution.)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:293T; B:A431; C:H1299) using a 7.5% SDS PAGE gel and PDI antibody at a dilution of 1:500)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:293T; B:A431; C:H1299) using a 7.5% SDS PAGE gel and PDI antibody at a dilution of 1:500)
IHC (Immunohiostchemistry)
(Immunohistochemical staining of paraffin-embedded H520 xenograft using PDI antibody at a dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemical staining of paraffin-embedded H520 xenograft using PDI antibody at a dilution of 1:100)
WB (Western Blot)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse liver) using a 7.5% SDS PAGE gel and PDI antibody at a dilution of 1:10000)
WB (Western Blot)
(Western blot analysis of 20 ug of whole cell lysate (A: mouse liver) using a 7.5% SDS PAGE gel and PDI antibody at a dilution of 1:10000)
Related Product Information for anti-PDI antibody
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Product Categories/Family for anti-PDI antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57 kDa
NCBI Official Full Name
protein disulfide isomerase, partial
NCBI Official Synonym Full Names
prolyl 4-hydroxylase subunit beta
NCBI Official Symbol
P4HB
NCBI Official Synonym Symbols
DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta
NCBI Protein Information
protein disulfide-isomerase
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Product Notes
The PDI (Catalog #AAA225109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDI can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PDI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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