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IP (Immunoprecipitation)
(PDZK1 was immunoprecipitated using:Lane A:0.5 mg Caco-2 Whole Cell Lysate4 uL anti-PDZK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDZK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :70 kDa)
Rabbit anti-Human PDZK1 Polyclonal Antibody | anti-PDZK1 antibody
Anti-PDZK1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PDZK1, Antibody; Anti-PDZK1 Antibody, Rabbit Polyclonal; PDZ domain containing 1; Anti-CAP70 Antibody; Anti-CLAMP Antibody; Anti-NHERF-3 Antibody; Anti-NHERF3 Antibody; Anti-PDZD1 Antibody; anti-PDZK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PDZK1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PDZK1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E coli-derived Human PDZK1 fragment
Preparation
Produced in rabbits immunized with E coli-derived Human PDZK1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PDZK1 was immunoprecipitated using:Lane A:0.5 mg Caco-2 Whole Cell Lysate4 uL anti-PDZK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDZK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :70 kDa)
IP (Immunoprecipitation)
(PDZK1 was immunoprecipitated using:Lane A:0.5 mg Caco-2 Whole Cell Lysate4 uL anti-PDZK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDZK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :70 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human PDZK1 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human PDZK1 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PDZK1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PDZK1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PDZK1 rabbit polyclonal antibody at 1:500 dilutionLane A: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:70 kDa)
WB (Western Blot)
(Anti-PDZK1 rabbit polyclonal antibody at 1:500 dilutionLane A: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:70 kDa)
Related Product Information for anti-PDZK1 antibody
PDZK1 (Post-synaptic density protein/Drosophila disc-large protein/Zonula occludens protein containing 1) is an adaptor protein that binds to the HDL receptor, scavenger receptor class B type I. PDZK1 is a multi-PDZ domain-containing adaptor protein that binds to the C terminus of the high-density lipoprotein receptor, scavenger receptor, class B, type I (SR-BI), and controls the posttranscriptional, tissue-specific expression of this lipoprotein receptor. PDZK1 (NHERF3) interacts with membrane proteins whereby modulating their spatial arrangement, membrane stability, and function. PDZK1 is important for maintaining adequate steady-state levels of SR-BI in the liver but is not essential for cell surface expression or function of hepatic SR-BI. Leukocyte PDZK1 deficiency in high fat-diet fed LDL receptor knockout mice has been found to increase atherosclerotic necrotic core formation and apoptosis of cells within atherosclerotic plaques.
References
Yu P, et al. (2018) Pdzk1 in leukocytes protects against cellular apoptosis and necrotic core development in atherosclerotic plaques in high fat diet fed ldl receptor deficient mice. Atherosclerosis 276 171-181.
Yu P, et al. (2018) Data on leukocyte pdzk1 deficiency affecting macrophage apoptosis but not monocyte recruitment, cell proliferation, macrophage abundance or er stress in atherosclerotic plaques of ldlr deficient mice. Data Brief 19 1148-1161.
Yesilaltay A, et al. (2006) Pdzk1 is required for maintaining hepatic scavenger receptor, class b, type i (sr-bi) steady state levels but not its surface localization or function. J Biol Chem 281 (39): 28975-28980.
Ferreira C, et al. (2019) The nuclear receptors pxr and lxr are regulators of the scaffold protein pdzk1. Biochim Biophys Acta Gene Regul Mech 1862 (4): 447-456.
Yu P, et al. (2018) Data on leukocyte pdzk1 deficiency affecting macrophage apoptosis but not monocyte recruitment, cell proliferation, macrophage abundance or er stress in atherosclerotic plaques of ldlr deficient mice. Data Brief 19 1148-1161.
Yesilaltay A, et al. (2006) Pdzk1 is required for maintaining hepatic scavenger receptor, class b, type i (sr-bi) steady state levels but not its surface localization or function. J Biol Chem 281 (39): 28975-28980.
Ferreira C, et al. (2019) The nuclear receptors pxr and lxr are regulators of the scaffold protein pdzk1. Biochim Biophys Acta Gene Regul Mech 1862 (4): 447-456.
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Product Notes
The PDZK1 (Catalog #AAA258987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDZK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDZK1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDZK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDZK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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