WB (Western Blot)
(Detection of human and mouse PGRMC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody AAA212774 (lot AAA212774-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse PGRMC1 Polyclonal Antibody | anti-PGRMC1 antibody
Rabbit anti-PGRMC1 Antibody, Affinity Purified
Gene Names
PGRMC1; MPR; HPR6.6
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PGRMC1, Antibody; Rabbit anti-PGRMC1 Antibody, Affinity Purified; membrane-associated progesterone receptor component 1; progesterone binding protein; MPR; HPR6.6; Dap1; IZA; Membrane-associated progesterone receptor component 1; progesterone receptor membrane component 1; anti-PGRMC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PGRMC1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Pig, Orangutan
Immunogen
Between 145 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PGRMC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody AAA212774 (lot AAA212774-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse PGRMC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody AAA212774 (lot AAA212774-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PGRMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody AAA212774 (lot AAA212774-1) used for IP at 6 ug per reaction. PGRMC1 was also immunoprecipitated by rabbit anti-PGRMC1 antibody BL17082. For blotting immunoprecipitated PGRMC1, AAA212774 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PGRMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody AAA212774 (lot AAA212774-1) used for IP at 6 ug per reaction. PGRMC1 was also immunoprecipitated by rabbit anti-PGRMC1 antibody BL17082. For blotting immunoprecipitated PGRMC1, AAA212774 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human PGRMC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PGRMC1 (Cat. No. AAA212774 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PGRMC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PGRMC1 (Cat. No. AAA212774 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-PGRMC1 antibody
Progesterone receptor membrane component 1 (PGRMC1) is a putative membrane-associated progesterone steroid receptor. PGRMC1 is expressed predominantly in the liver and kidne [taken from NCBI Entrez Gene (Gene ID: 10857)].
Product Categories/Family for anti-PGRMC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,879 Da
NCBI Official Full Name
membrane-associated progesterone receptor component 1 isoform 1
NCBI Official Synonym Full Names
progesterone receptor membrane component 1
NCBI Official Symbol
PGRMC1
NCBI Official Synonym Symbols
MPR; HPR6.6
NCBI Protein Information
membrane-associated progesterone receptor component 1; progesterone binding protein
UniProt Protein Name
Membrane-associated progesterone receptor component 1
UniProt Gene Name
PGRMC1
UniProt Synonym Gene Names
HPR6.6; PGRMC; mPR
UniProt Entry Name
PGRC1_HUMAN
Similar Products
Product Notes
The PGRMC1 pgrmc1 (Catalog #AAA212774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PGRMC1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PGRMC1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PGRMC1 pgrmc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGRMC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.