Application Data
(The involvement of AMPK and Akt in the negative regulation of mTOR by APPL1 in STZ-induced diabetic rats.The variations of p-AMPK and p-Akt in normal control rats with vacant lentiviral vector control, normal control rats with APPL1 knockdown, PDN rats, and PDN rats with APPL1 knockdown, and PDN rats with APPL1 overexpression.The abbreviations for the groups of negative control, normal control + shRNA encoding APPL1, painful diabetic neuropathy (PDN), PDN + shRNA encoding APPLL (shRNA) and PDN + APPL1 genetic overexpression are shown as CON, CON + shRNA, PDN, PDN + shRNA, and PDN + OXP. (n = 4, *P < 0.05 vs. NC group, # P < 0.05 vs. PDN group).Data are expressed as the means ± SEM.)
Rabbit Phospho-AMPK1 (Ser486) Polyclonal Antibody | anti-AMPK1 antibody
Phospho-AMPK1 (Ser486) Antibody
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
Application Data
(The involvement of AMPK and Akt in the negative regulation of mTOR by APPL1 in STZ-induced diabetic rats.The variations of p-AMPK and p-Akt in normal control rats with vacant lentiviral vector control, normal control rats with APPL1 knockdown, PDN rats, and PDN rats with APPL1 knockdown, and PDN rats with APPL1 overexpression.The abbreviations for the groups of negative control, normal control + shRNA encoding APPL1, painful diabetic neuropathy (PDN), PDN + shRNA encoding APPLL (shRNA) and PDN + APPL1 genetic overexpression are shown as CON, CON + shRNA, PDN, PDN + shRNA, and PDN + OXP. (n = 4, *P < 0.05 vs. NC group, # P < 0.05 vs. PDN group).Data are expressed as the means ± SEM.)
Application Data
(The involvement of AMPK and Akt in the negative regulation of mTOR by APPL1 in STZ-induced diabetic rats.The variations of p-AMPK and p-Akt in normal control rats with vacant lentiviral vector control, normal control rats with APPL1 knockdown, PDN rats, and PDN rats with APPL1 knockdown, and PDN rats with APPL1 overexpression.The abbreviations for the groups of negative control, normal control + shRNA encoding APPL1, painful diabetic neuropathy (PDN), PDN + shRNA encoding APPLL (shRNA) and PDN + APPL1 genetic overexpression are shown as CON, CON + shRNA, PDN, PDN + shRNA, and PDN + OXP. (n = 4, *P < 0.05 vs. NC group, # P < 0.05 vs. PDN group).Data are expressed as the means ± SEM.)
ELISA (Peptide-ELISA)
(peptide-ELISA analysis of AAA31070.showing specificity to antigen peptide.Peptides concentration: 1ug/ml.P-peptide: phospho-peptide.N-peptide: non-phospho-peptide.)
ELISA (Peptide-ELISA)
(peptide-ELISA analysis of AAA31070.showing specificity to antigen peptide.Peptides concentration: 1ug/ml.P-peptide: phospho-peptide.N-peptide: non-phospho-peptide.)
IF (Immunofluorescence)
(AAA31070 staining HepG2 cells(4h of LPS treatment) by IF/ICC.The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C.Samples were then incubated with primary Ab( AAA31070 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C.An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA31070 staining HepG2 cells(4h of LPS treatment) by IF/ICC.The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C.Samples were then incubated with primary Ab( AAA31070 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C.An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohistochemistry)
(AAA31070 at 1/100 staining Rat ovary tissue by IHC-P.The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The sample was then blocked and incubated with the primary antibody at 4°C overnight.An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA31070 at 1/100 staining Rat ovary tissue by IHC-P.The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The sample was then blocked and incubated with the primary antibody at 4°C overnight.An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistchemistry)
(AAA31070 at 1/100 staining Mouse kidney tissue by IHC-P.The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The sample was then blocked and incubated with the primary antibody at 4°C overnight.An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistchemistry)
(AAA31070 at 1/100 staining Mouse kidney tissue by IHC-P.The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The sample was then blocked and incubated with the primary antibody at 4°C overnight.An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA31070 at 1/200 staining human colon cancer tissue sections by IHC-P.The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C.An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA31070 at 1/200 staining human colon cancer tissue sections by IHC-P.The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C.An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-AMPK1 (Ser486) Antibody.Lane 1: Rat lung, blocked with antigen-specific peptides,Lane 2: Rat lung,Lane 3: B16F10 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-AMPK1 (Ser486) Antibody.Lane 1: Rat lung, blocked with antigen-specific peptides,Lane 2: Rat lung,Lane 3: B16F10 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-AMPK1 (Ser486) Antibody.Lane 1: H2O2 treated EC304 cells, blocked with antigen-specific peptides,Lane 2: H2O2 treated EC304 cells,Lane 3: UV treated NIH-3T3 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-AMPK1 (Ser486) Antibody.Lane 1: H2O2 treated EC304 cells, blocked with antigen-specific peptides,Lane 2: H2O2 treated EC304 cells,Lane 3: UV treated NIH-3T3 cells.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using Phospho-AMPK1 (Ser486) Antibody.The lane on the left was treated with blocking peptide.Observed bands: 70 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using Phospho-AMPK1 (Ser486) Antibody.The lane on the left was treated with blocking peptide.Observed bands: 70 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-AMPK1 (Ser486) Antibody.Lane 1: Rat spleen lysates;Lane 2: Rat liver lysates;Lane 3: Rat liver lysates treated with blocking peptide;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-AMPK1 (Ser486) Antibody.Lane 1: Rat spleen lysates;Lane 2: Rat liver lysates;Lane 3: Rat liver lysates treated with blocking peptide;)
NCBI and Uniprot Product Information
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Product Notes
The AMPK1 prkaa1 (Catalog #AAA31070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-AMPK1 (Ser486) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-AMPK1 (Ser486) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofoluroescence (IF)/Immunocytochemistry (ICC). WB: 1:500-1:2000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500 *The optimal dilutions should be determined by the end user. Researchers should empirically determine the suitability of the AMPK1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-AMPK1 (Ser486), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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