Application Data
Rabbit LRP6 (phospho) Antibody | anti-LRP6 (phospho) antibody
Rabbit anti LRP6 Phospho-specific antibody
Gene Names
LRP6; ADCAD2; FLJ90062; FLJ90421
Reactivity
Human, Rat, Mouse, Bovine, Canine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
LRP6 (phospho); N/A; Rabbit anti LRP6 Phospho-specific antibody; Rabbit anti-LRP6 (pT1479); Low-density lipoprotein Receptor-related Protein 6 (LRP6); anti-LRP6 (phospho) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Canine
Specificity
This antibody recognizes ~180 kDa of human LRP6 protein. It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-LRP6 (phospho) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
A synthetic peptide corresponding to the epitope KGTYF at a phosphorylation threonine1479 in LRP6 protein
Positive Control
Conditioned 3T3 cell lysate
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Application Data
Application Data
Related Product Information for anti-LRP6 (phospho) antibody
Low-desnsity lipoprotein receptor (LDLR)-related protein 6 (LRP6) is a member of the LDLR family which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LRP5 cuases several human diseases including bone mineral density variability1, endosteal hyperostosis, autosomal dominant osteopetrosis type I etc. The V171 mutation in LRP5 causes high bone density by impairing the action of a normal antagonist of the Wnt pathway and thus increasing Wnt signaling. The activity of LRP5 and LRP6 can be inhibited by binding of some members of Dickkopf (DKK) family proteins. Upon stimulation with Wnt, LRP5 and LRP6 are phosphorylated at multiple sites including Thr 1479, Ser 1490 and Thr 1492 by kinases such as GSK-3 and CK1.
References
Zahid Khan et al. (2007), Molecular and Cellular Biology, 27 (20), 7291-7301
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
180 kDa
NCBI Official Full Name
low-density lipoprotein receptor-related protein 6
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein 6
NCBI Official Symbol
LRP6
NCBI Official Synonym Symbols
ADCAD2; FLJ90062; FLJ90421
NCBI Protein Information
low-density lipoprotein receptor-related protein 6; LRP-6; OTTHUMP00000238979; OTTHUMP00000238980; OTTHUMP00000238982
UniProt Protein Name
Low-density lipoprotein receptor-related protein 6
UniProt Gene Name
LRP6
UniProt Entry Name
LRP6_HUMAN
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Product Notes
The LRP6 (phospho) lrp6 (Catalog #AAA14084) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti LRP6 Phospho-specific antibody reacts with Human, Rat, Mouse, Bovine, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's LRP6 (phospho) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the LRP6 (phospho) lrp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRP6 (phospho), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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