WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-IKKγ-S376 pAb at 1:1000 dilution or IKKγ antibody (A0917).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Rabbit Phospho-IKKgamma-S376 Polyclonal Antibody | anti-IKKgamma-S376 antibody
Phospho-IKKgamma-S376 Rabbit pAb
Gene Names
IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
Phospho-IKKgamma-S376, Antibody; Phospho-IKKgamma-S376 Rabbit pAb; IKBKG; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9; NF-kappa-B essential modulator; anti-IKKgamma-S376 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-IKKgamma-S376 antibody
WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding S376 of human IKKgamma.
Cellular Location
Cytoplasm, Nucleus
Positive Samples
NIH/3T3, C6
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-IKKγ-S376 pAb at 1:1000 dilution or IKKγ antibody (A0917).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using Phospho-IKKγ-S376 pAb at 1:1000 dilution or IKKγ antibody (A0917).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Phospho-IKKγ-S376 antibody at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Phospho-IKKγ-S376 antibody at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Related Product Information for anti-IKKgamma-S376 antibody
Background: This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
Product Categories/Family for anti-IKKgamma-S376 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,198 Da
NCBI Official Full Name
NF-kappa-B essential modulator isoform b
NCBI Official Synonym Full Names
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
NCBI Official Symbol
IKBKG
NCBI Official Synonym Symbols
IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma
NCBI Protein Information
NF-kappa-B essential modulator; IKKG; IKKAP1; incontinentia pigmenti; IkB kinase gamma subunit; ikB kinase subunit gamma; NFkappaB essential modulator; NF-kappa-B essential modifier; I-kappa-B kinase subunit gamma; ikB kinase-associated protein 1; inhibit
UniProt Protein Name
NF-kappa-B essential modulator
UniProt Gene Name
IKBKG
UniProt Synonym Gene Names
FIP3; NEMO; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG
UniProt Entry Name
NEMO_HUMAN
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Product Notes
The IKKgamma-S376 ikbkg (Catalog #AAA282130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-IKKgamma-S376 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-IKKgamma-S376 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the IKKgamma-S376 ikbkg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-IKKgamma-S376, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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