IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit Phospho-MAPKAPK2 (S272) Polyclonal Antibody | anti-MAPKAPK2 antibody
Phospho-MAPKAPK2 (S272) Antibody
Gene Names
MAPKAPK2; MK2; MK-2; MAPKAP-K2
Reactivity
Human (Predicted Reactivity: Mouse, Rabbit)
Applications
Immunohistochemistry, ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-MAPKAPK2 (S272), Antibody; Phospho-MAPKAPK2 (S272) Antibody; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2; anti-MAPKAPK2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAPKAPK2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human MAPKAPK2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Length
370
Applicable Applications for anti-MAPKAPK2 antibody
IHC (Immunohistochemistry), ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
DB (Dot Blot)
(Dot blot analysis of anti-Phospho-MAPKAPK2-S272 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-Phospho-MAPKAPK2-S272 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-MAPKAPK2 antibody
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Product Categories/Family for anti-MAPKAPK2 antibody
References
Lukas, S.M., et al., Biochemistry 43(31):9950-9960 (2004).
Underwood, K.W., et al., Structure (Camb.) 11(6):627-636 (2003).
Meng, W., et al., J. Biol. Chem. 277(40):37401-37405 (2002).
Han, Q., et al., J. Biol. Chem. 277(50):48379-48385 (2002).
Werz, O., et al., Proc. Natl. Acad. Sci. U.S.A. 97(10):5261-5266 (2000).
Underwood, K.W., et al., Structure (Camb.) 11(6):627-636 (2003).
Meng, W., et al., J. Biol. Chem. 277(40):37401-37405 (2002).
Han, Q., et al., J. Biol. Chem. 277(50):48379-48385 (2002).
Werz, O., et al., Proc. Natl. Acad. Sci. U.S.A. 97(10):5261-5266 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45568
NCBI Official Full Name
MAP kinase-activated protein kinase 2 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase-activated protein kinase 2
NCBI Official Symbol
MAPKAPK2
NCBI Official Synonym Symbols
MK2; MK-2; MAPKAP-K2
NCBI Protein Information
MAP kinase-activated protein kinase 2
UniProt Protein Name
MAP kinase-activated protein kinase 2
UniProt Gene Name
MAPKAPK2
UniProt Synonym Gene Names
MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2
UniProt Entry Name
MAPK2_HUMAN
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Product Notes
The MAPKAPK2 mapkapk2 (Catalog #AAA285365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MAPKAPK2 (S272) Antibody reacts with Human (Predicted Reactivity: Mouse, Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-MAPKAPK2 (S272) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the MAPKAPK2 mapkapk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-MAPKAPK2 (S272), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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