IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human Phospho-p27Kip1 (T157) Polyclonal Antibody | anti-CDKN1B antibody
Phospho-p27Kip1 (T157) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-p27Kip1 (T157), Antibody; Phospho-p27Kip1 (T157) Antibody; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; CDKN1B; KIP1; anti-CDKN1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
198
Applicable Applications for anti-CDKN1B antibody
IHC (Immunohistochemistry), ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
DB (Dot Blot)
(Dot blot analysis of anti-p27Kip1-T157 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-p27Kip1-T157 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Related Product Information for anti-CDKN1B antibody
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Product Categories/Family for anti-CDKN1B antibody
References
Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005).
Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).
Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).
Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22073
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1B
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1B
UniProt Gene Name
CDKN1B
UniProt Synonym Gene Names
KIP1
UniProt Entry Name
CDN1B_HUMAN
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Product Notes
The CDKN1B cdkn1b (Catalog #AAA290167) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-p27Kip1 (T157) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-p27Kip1 (T157) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the CDKN1B cdkn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-p27Kip1 (T157), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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