DB (Dot Blot)
(Dot blot analysis of anti-PAK1-pT423 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit Phospho-PAK1 (T423) Polyclonal Antibody | anti-PAK1 antibody
Phospho-PAK1 (T423) Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat, Rabbit, Xenopus)
Applications
ELISA, Dot Blot, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-PAK1 (T423), Antibody; Phospho-PAK1 (T423) Antibody; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK1; anti-PAK1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat, Rabbit, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PAK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T423 of human PAK1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.46 (varies by lot)
Sequence Length
553
Applicable Applications for anti-PAK1 antibody
ELISA, DB (Dot Blot), IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-PAK1-pT423 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-PAK1-pT423 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Phospho-PAK1-pT423 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Phospho-PAK1-pT423 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-PAK1 antibody
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
Product Categories/Family for anti-PAK1 antibody
References
Li, W., et al., J. Biol. Chem. 279(31):32824-32831 (2004).
DerMardirossian, C., et al., Mol. Cell 15(1):117-127 (2004).
Weber, D.S., et al., Circ. Res. 94(9):1219-1226 (2004).
Balasenthil, S., et al., J. Biol. Chem. 279(2):1422-1428 (2004).
Wu, R.F., et al., J. Virol. 78(2):779-789 (2004).
DerMardirossian, C., et al., Mol. Cell 15(1):117-127 (2004).
Weber, D.S., et al., Circ. Res. 94(9):1219-1226 (2004).
Balasenthil, S., et al., J. Biol. Chem. 279(2):1422-1428 (2004).
Wu, R.F., et al., J. Virol. 78(2):779-789 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60647
NCBI Official Full Name
serine/threonine-protein kinase PAK 1 isoform 1
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 1
NCBI Official Symbol
PAK1
NCBI Official Synonym Symbols
PAKalpha
NCBI Protein Information
serine/threonine-protein kinase PAK 1
UniProt Protein Name
Serine/threonine-protein kinase PAK 1
UniProt Gene Name
PAK1
UniProt Synonym Gene Names
PAK-1
UniProt Entry Name
PAK1_HUMAN
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Product Notes
The PAK1 pak1 (Catalog #AAA288458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PAK1 (T423) Antibody reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat, Rabbit, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-PAK1 (T423) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PAK1 pak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PAK1 (T423), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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