WB (Western Blot)
(Western blot analysis of lysates from HeLa cells, using Phospho-PLN-T17 Rabbit pAb (AAA282120) at 1:400 dilution. HeLa cells were treated by A-PP mixed solution (1ul) at 30 degree C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit Phospho-PLN-T17 Polyclonal Antibody | anti-PLN-T17 antibody
Phospho-PLN-T17 Rabbit pAb
Gene Names
PLN; PLB; CMD1P; CMH18
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
Phospho-PLN-T17, Antibody; Phospho-PLN-T17 Rabbit pAb; CMD1P; CMH18; PLB; PLN; anti-PLN-T17 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Applicable Applications for anti-PLN-T17 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic phosphorylated peptide around T17 of human PLN (NP_002658.1).
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells, using Phospho-PLN-T17 Rabbit pAb (AAA282120) at 1:400 dilution. HeLa cells were treated by A-PP mixed solution (1ul) at 30 degree C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells, using Phospho-PLN-T17 Rabbit pAb (AAA282120) at 1:400 dilution. HeLa cells were treated by A-PP mixed solution (1ul) at 30 degree C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human appendix using Phospho-PLN-T17 Rabbit pAb (AAA282120) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human appendix using Phospho-PLN-T17 Rabbit pAb (AAA282120) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
Related Product Information for anti-PLN-T17 antibody
Background: The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]
Product Categories/Family for anti-PLN-T17 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW: 24kDa
Calculated MW: 6kDa
Calculated MW: 6kDa
NCBI Official Full Name
cardiac phospholamban
NCBI Official Synonym Full Names
phospholamban
NCBI Official Symbol
PLN
NCBI Official Synonym Symbols
PLB; CMD1P; CMH18
NCBI Protein Information
cardiac phospholamban
UniProt Protein Name
Cardiac phospholamban
UniProt Gene Name
PLN
UniProt Synonym Gene Names
PLB; PLB
UniProt Entry Name
PPLA_HUMAN
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Product Notes
The PLN-T17 pln (Catalog #AAA282120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospho-PLN-T17 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLN-T17 pln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PLN-T17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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