DB (Dot Blot)
(Dot blot analysis of anti-RASGRF1-pS929 Pab (RB14017) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit anti-Human Phospho-RASGRF1 Polyclonal Antibody | anti-RASGRF1 antibody
Phospho-RASGRF1 (S929) Antibody
Gene Names
RASGRF1; GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
Reactivity
Human
Applications
Dot Blot, Western Blot
Synonyms
Phospho-RASGRF1, Antibody; Phospho-RASGRF1 (S929) Antibody; Affinity Purified Rabbit Polyclonal Antibody (Pab); Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25; RASGRF1; CDC25; GRF1; anti-RASGRF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB14017
Specificity
This RASGRF1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S929 of human RASGRF1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-RASGRF1 antibody
DB (Dot Blot), WB (Western Blot)
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-RASGRF1-pS929 Pab (RB14017) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-RASGRF1-pS929 Pab (RB14017) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with Forskolin(50uM, 30min), using Phospho-RASGRF1(S929) Antibody(upper) or Tubulin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with Forskolin(50uM, 30min), using Phospho-RASGRF1(S929) Antibody(upper) or Tubulin (lower).)
Related Product Information for anti-RASGRF1 antibody
RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
Product Categories/Family for anti-RASGRF1 antibody
References
Iguchi,T., Int. J. Oncol. 31 (2), 285-291 (2007)Holmes,R., Mol. Cell. Biol. 26 (24), 9564-9570 (2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
145234
NCBI Official Full Name
ras-specific guanine nucleotide-releasing factor 1 isoform 2
NCBI Official Synonym Full Names
Ras protein-specific guanine nucleotide-releasing factor 1
NCBI Official Symbol
RASGRF1
NCBI Official Synonym Symbols
GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
NCBI Protein Information
ras-specific guanine nucleotide-releasing factor 1
UniProt Protein Name
Ras-specific guanine nucleotide-releasing factor 1
UniProt Gene Name
RASGRF1
UniProt Synonym Gene Names
CDC25; GNRP; GRF1; Ras-GRF1; GNRP
UniProt Entry Name
RGRF1_HUMAN
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Product Notes
The RASGRF1 rasgrf1 (Catalog #AAA290977) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-RASGRF1 (S929) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-RASGRF1 can be used in a range of immunoassay formats including, but not limited to, DB (Dot Blot), WB (Western Blot). Researchers should empirically determine the suitability of the RASGRF1 rasgrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-RASGRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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