Rabbit anti-Human Phospho-RB (S612) Polyclonal Antibody | anti-RB1 antibody

Phospho-RB (S612) Antibody

Cat. Num. AAA287806

• Gene Names: RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-RB (S612), Antibody; Phospho-RB (S612) Antibody; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1; anti-RB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S612 of human RB.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 928

• Applicable Applications for anti-RB1 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed: ). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed: ). Both physically blocks E2Fs transactivating domain and recruits chromatin- modifying enzymes that actively repress transcription (PubMed: ). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed: ). RB1 also promotes the G0-G1 transition upon phosphorylation and activation byCDK3/cyclin-C (PubMed: ). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1- dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).
• Cellular Location: Nucleus. Note=During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization.
• Tissue Location: Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) (PubMed:20940255)
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from Hela cell line, untreated or treated with UV(2h), using Phospho-RB(S612) Antibody(upper) or Tubulin (lower).)

WB (Western Blot)

(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with UV(2h), using Phospho-RB(S612) Antibody(upper) or Tubulin (lower).)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma)

WB (Western Blot)

(The anti-RB Pab is used in Western blot to detect RB in, from left to right, HL60, T47D, and SK-BR-3 tissue lysates.)

Related Product Information for anti-RB1 antibody

Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.[supplied by OMIM].

Product Categories/Family for anti-RB1 antibody

Cancer; Cell Biology; Phospho Antibodies

References

Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004). Cui, X., et al., Hum. Pathol. 35(10):1189-1195 (2004). Borah, S., et al., J. Virol. 78(19):10336-10347 (2004). Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004). Lohmann, D.R., et al., J. Biol. Chem. 129(1):23-28 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 108773787
• NCBI GeneID: 5925
• NCBI Accession #: NP_000312.2
• NCBI GenBank Nucleotide #: NM_000321.2
• UniProt Accession #: P06400; P78499; Q5VW46; Q8IZL4; A8K5E3
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: retinoblastoma 1
• NCBI Official Symbol: RB1
• NCBI Official Synonym Symbols: RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• NCBI Protein Information: retinoblastoma-associated protein
• UniProt Protein Name: Retinoblastoma-associated protein
• UniProt Gene Name: RB1
• UniProt Synonym Gene Names: Rb
• UniProt Entry Name: RB_HUMAN