DB (Dot Blot)
(Dot blot analysis of Phospho-SNAP25-pT138 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit Phospho-SNAP25 (T138) Polyclonal Antibody | anti-SNAP25 antibody
Phospho-SNAP25 (T138) Antibody
Gene Names
SNAP25; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Reactivity
Human (Predicted Reactivity: Zebrafish, Bovine, Chicken, Mouse, Rat)
Applications
ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-SNAP25 (T138), Antibody; Phospho-SNAP25 (T138) Antibody; Synaptosomal-associated protein 25; SNAP-25; Super protein; SUP; Synaptosomal-associated 25 kDa protein; SNAP25; SNAP; anti-SNAP25 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Zebrafish, Bovine, Chicken, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SNAP25 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T138 of human SNAP25.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
206
Applicable Applications for anti-SNAP25 antibody
ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
t-SNARE involved in the molecular regulationof neurotransmitter release. May play animportant role in the synaptic function ofspecific neuronal systems. Associates withproteins involved in vesicle docking andmembrane fusion. Regulates plasmamembrane recycling through its interactionwith CENPF. Modulates the gatingcharacteristics of the delayed rectifiervoltage-dependent potassium channel KCNB1in pancreatic beta cells.
Cellular Location
Cytoplasm, perinuclear region{ECO:0000250|UniProtKB:P60879}. Cellmembrane{ECO:0000250|UniProtKB:P60881};Lipid-anchor{ECO:0000250|UniProtKB:P60879}. Celljunction, synapse, synaptosome{ECO:0000250|UniProtKB:P60879}.Photoreceptor inner segment{ECO:0000250|UniProtKB:P60879}.Note=Membrane association requirespalmitoylation. Expressed throughoutcytoplasm, concentrating at the perinuclearregion. Colocalizes with KCNB1 at the cellmembrane (By similarity). Colocalizes withPLCL1 at the cell membrane (By similarity).{ECO:0000250|UniProtKB:P60879,ECO:0000250|UniProtKB:P60881}
Tissue Location
Neurons of the neocortex, hippocampus,piriform cortex, anterior thalamic nuclei,pontine nuclei, and granule cells of thecerebellum.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of Phospho-SNAP25-pT138 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
DB (Dot Blot)
(Dot blot analysis of Phospho-SNAP25-pT138 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Related Product Information for anti-SNAP25 antibody
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Product Categories/Family for anti-SNAP25 antibody
References
Mohrmann, R., et al. Science 330(6003):502-505(2010)
Tsai, Y.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(38):16554-16559(2010)
Condliffe, S.B., et al. J. Biol. Chem. 285(32):24968-24976(2010)
Weber, J.P., et al. EMBO J. 29(15):2477-2490(2010)
Walter, A.M., et al. J. Cell Biol. 188(3):401-413(2010)
Tsai, Y.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(38):16554-16559(2010)
Condliffe, S.B., et al. J. Biol. Chem. 285(32):24968-24976(2010)
Weber, J.P., et al. EMBO J. 29(15):2477-2490(2010)
Walter, A.M., et al. J. Cell Biol. 188(3):401-413(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23315
NCBI Official Full Name
synaptosomal-associated protein 25 isoform SNAP25A
NCBI Official Synonym Full Names
synaptosomal-associated protein, 25kDa
NCBI Official Symbol
SNAP25
NCBI Official Synonym Symbols
RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
NCBI Protein Information
synaptosomal-associated protein 25
UniProt Protein Name
Synaptosomal-associated protein 25
UniProt Gene Name
SNAP25
UniProt Synonym Gene Names
SNAP; SNAP-25; SUP
UniProt Entry Name
SNP25_HUMAN
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Product Notes
The SNAP25 snap25 (Catalog #AAA289167) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SNAP25 (T138) Antibody reacts with Human (Predicted Reactivity: Zebrafish, Bovine, Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-SNAP25 (T138) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-SNAP25 (T138), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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