IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Phospho-TSC2-S1387 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit Phospho-TSC2 (S1387) Polyclonal Antibody | anti-TSC2 antibody
Phospho-TSC2 (S1387) Antibody
Gene Names
TSC2; LAM; TSC4; PPP1R160
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-TSC2 (S1387), Antibody; Phospho-TSC2 (S1387) Antibody; Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4; anti-TSC2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TSC2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1387 of human TSC2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Sequence Length
239
Applicable Applications for anti-TSC2 antibody
IF (Immunofluorescence), ELISA, DB (Dot Blot)
Function
In complex with TSC1, this tumor suppressor inhibits the nutrients-mediated of growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.
Cellular Location
Cytoplasm. Membrane; Peripheral membrane protein. Note= At steady state found in association with membranes.
Tissue Location
Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Phospho-TSC2-S1387 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Phospho-TSC2-S1387 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
DB (Dot Blot)
(Dot blot analysis of Phospho-TSC2-S1387 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
DB (Dot Blot)
(Dot blot analysis of Phospho-TSC2-S1387 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Related Product Information for anti-TSC2 antibody
Mutations in TSC2 lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.
Product Categories/Family for anti-TSC2 antibody
References
Li, Y., et al., Mol. Cell. Biol. 24(18):7965-7975 (2004).
Karbowniczek, M., et al., J. Biol. Chem. 279(29):29930-29937 (2004).
Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004).
Birchenall-Roberts, M.C., et al., J. Biol. Chem. 279(24):25605-25613 (2004).
Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).
Karbowniczek, M., et al., J. Biol. Chem. 279(29):29930-29937 (2004).
Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004).
Birchenall-Roberts, M.C., et al., J. Biol. Chem. 279(24):25605-25613 (2004).
Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
200608
NCBI Official Full Name
tuberin isoform 1
NCBI Official Synonym Full Names
tuberous sclerosis 2
NCBI Official Symbol
TSC2
NCBI Official Synonym Symbols
LAM; TSC4; PPP1R160
NCBI Protein Information
tuberin
UniProt Protein Name
Tuberin
UniProt Gene Name
TSC2
UniProt Synonym Gene Names
TSC4
UniProt Entry Name
TSC2_HUMAN
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Product Notes
The TSC2 tsc2 (Catalog #AAA287625) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TSC2 (S1387) Antibody reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-TSC2 (S1387) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the TSC2 tsc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-TSC2 (S1387), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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