WB (Western Blot)
(Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit PI3-kinase p85-alpha/gamma Polyclonal Antibody | anti-PIK3R1 antibody
PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
PI3-kinase p85-alpha/gamma, Antibody; PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody; GRB1; P85A; PI3-kinase p85-alpha subunit; PI3K; PI3K p85-alpha; Phosphatidylinositol 3-kinase regulatory alpha subunit; PtdIns-3-kinase p85-alpha; P55G; PI3-kinase p85-gamma subunit; PI3K p85-gamma; Phosphatidylinositol 3-kinase regulatory gamma subunit; anti-PIK3R1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody detects endogenous levels of PI3-kinase p85-alpha/gamma only when phosphorylated at Tyr467/199.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
361
Applicable Applications for anti-PIK3R1 antibody
ELISA, IF (Immunofluorescence), WB (Western Blot)
Target Modification
Phospho
Modification Sites
H:Y467/Y199; M:Y467/Y199; R:Y467/Y199
Immunogen
The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199.
Immunogen Range
436-485
Preparation and Storage
Stable at -20 degree C for at least 1 year.
WB (Western Blot)
(Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.)
WB (Western Blot)
(Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83 kDa
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 1
NCBI Official Symbol
PIK3R1
NCBI Official Synonym Symbols
p85; AGM7; GRB1; IMD36; p85-ALPHA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Gene Name
PIK3R1
UniProt Synonym Gene Names
GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
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Product Notes
The PIK3R1 pik3r1 (Catalog #AAA316442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PI3-kinase p85-alpha/gamma can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PIK3R1 pik3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3-kinase p85-alpha/gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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