Rabbit PI4KCA Polyclonal Antibody | anti-PI4KCA antibody

PI4KCA Antibody (N-term)

Cat. Num. AAA290984

• Gene Names: PI4KA; PIK4CA; pi4K230; PI4K-ALPHA
• Reactivity: Human; Predicted Reactivity: Bovine, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: PI4KCA, Antibody; PI4KCA Antibody (N-term); Purified Rabbit Polyclonal Antibody (Pab); Phosphatidylinositol 4-kinase alpha; PI4-kinase alpha; PI4K-alpha; PtdIns-4-kinase alpha; PI4KA; PIK4; PIK4CA; anti-PI4KCA antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB1671-1672
• Specificity: This PI4KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human PI4KCA.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Applicable Applications for anti-PI4KCA antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Antigen Source: Human
• Antigen Region: 6-36
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PI4KCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(The anti-PI4KCA Pab is used in Western blot to detect PI4KCA in human placenta tissue lysate.)

Related Product Information for anti-PI4KCA antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein.By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. Two transcript variants encoding different isoforms have been described for this gene. Variant 1 is alternatively spliced at the 5' end compared to transcript variant 2. However, it maintains the same reading frame and encodes an isoform 1 (97 kDa) which is truncated at the N-terminus compared to isoform 2 (230 kDa). Isoform 1 has enzymatic properties characteristic of type II PI 4-kinases.Variant 2 is full-length, and encodes the longer isoform 2 with a different N-terminus compared to isoform 1. Isoform 2 has enzymatic properties characteristic of type III PI 4-kinases.

Product Categories/Family for anti-PI4KCA antibody

Primary Antibodies; Signal Transduction

References

Subrahmanyam, G., et al., Eur. J. Immunol. 33(1):46-52 (2003).Huang, C., et al., J. Biol. Chem. 277(23):20293-20300 (2002).Gehrmann, T., et al., Biochim. Biophys. Acta 1437(3):341-356 (1999).Gehrmann, T., et al., Eur. J. Biochem. 253(2):357-370 (1998).Wong, K., et al., J. Biol. Chem. 269(46):28878-28884 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 155030227
• NCBI GeneID: 5297
• NCBI Accession #: NP_002641.1
• NCBI GenBank Nucleotide #: NM_002650
• UniProt Accession #: P42356; O08662; O02811; E9Q3L2
• Molecular Weight: ~120 kDa
• NCBI Official Full Name: Homo sapiens phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: phosphatidylinositol 4-kinase, catalytic, alpha
• NCBI Official Symbol: PI4KA
• NCBI Official Synonym Symbols: PIK4CA; pi4K230; PI4K-ALPHA
• NCBI Protein Information: phosphatidylinositol 4-kinase alpha; PI4-kinase alpha; ptdIns-4-kinase alpha; phosphatidylinositol 4-kinase 230; phosphatidylinositol 4-kinase, type III, alpha
• UniProt Protein Name: Phosphatidylinositol 4-kinase alpha
• UniProt Gene Name: PI4KA
• UniProt Synonym Gene Names: PIK4; PIK4CA; PI4-kinase alpha; PI4K-alpha; PtdIns-4-kinase alpha
• UniProt Entry Name: PI4KA_HUMAN