IP (Immunoprecipitation)
(EKLF was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Raw264.7 Whole Cell Lysate4 uL anti-EKLF rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EKLF rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
Rabbit PIK3R1 Polyclonal Antibody | anti-PIK3R1 antibody
Anti-PIK3R1 Antibody, Rabbit Polyclonal
Gene Names
PIK3R1; p85; AGM7; GRB1; p85-ALPHA
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PIK3R1, Antibody; Anti-PIK3R1 Antibody, Rabbit Polyclonal; PIK3R1 Antibody, Rabbit PAb, Antigen Affinity Purified; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); Anti-AGM7 Antibody; Anti-GRB1 Antibody; Anti-IMD36 Antibody; Anti-p85 Antibody; Anti-p85-ALPHA Antibody; anti-PIK3R1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PIK3R1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PIK3R1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human PIK3R1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PIK3R1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(EKLF was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Raw264.7 Whole Cell Lysate4 uL anti-EKLF rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EKLF rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
IP (Immunoprecipitation)
(EKLF was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Raw264.7 Whole Cell Lysate4 uL anti-EKLF rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EKLF rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
WB (Western Blot)
(Anti-PIK3R1 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: HT29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:38 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PIK3R1 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: HT29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:38 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PIK3R1 antibody
Phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), which encodes protein PI3Kp85 (p85), was identified as a FOXA1 target gene. As a tumor suppressor, FOXA1 targets PIK3R1 directly to inhibit PI3K/Akt signaling pathway, thus exerting a negative regulatory effect on proliferation, migration, and invasion of HCC in male patients.
Product Categories/Family for anti-PIK3R1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,598 Da
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
NCBI Official Symbol
PIK3R1
NCBI Official Synonym Symbols
p85; AGM7; GRB1; p85-ALPHA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alph
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Gene Name
PIK3R1
UniProt Synonym Gene Names
GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha
UniProt Entry Name
P85A_HUMAN
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Product Notes
The PIK3R1 pik3r1 (Catalog #AAA254611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PIK3R1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PIK3R1 pik3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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