IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human PIK3R3 Polyclonal Antibody | anti-PIK3R3 antibody
PIK3R3 Antibody (C-term)
Gene Names
PIK3R3; p55; p55PIK; p55-GAMMA
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PIK3R3, Antibody; PIK3R3 Antibody (C-term); Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK; PIK3R3; anti-PIK3R3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
316-346
Sequence Length
366
Applicable Applications for anti-PIK3R3 antibody
IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with PIK3R3 Antibody(C-term). AAA288493 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with PIK3R3 Antibody(C-term). AAA288493 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-PIK3R3 antibody
PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.
Product Categories/Family for anti-PIK3R3 antibody
References
Dey, B.R., et al., Gene 209 (1-2), 175-183 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54448
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit gamma
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
NCBI Official Symbol
PIK3R3
NCBI Official Synonym Symbols
p55; p55PIK; p55-GAMMA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit gamma
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit gamma
UniProt Gene Name
PIK3R3
UniProt Synonym Gene Names
PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma
UniProt Entry Name
P55G_HUMAN
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Product Notes
The PIK3R3 pik3r3 (Catalog #AAA288493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 316-346. The PIK3R3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PIK3R3 pik3r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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