WB (Western Blot)
(Western blot analysis of extracts of Mouse lung, using PIKFYVE antibody (AAA37642) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
Rabbit anti-Human, Mouse PIKFYVE Polyclonal Antibody | anti-PIKFYVE antibody
PIKFYVE Polyclonal Antibody
Gene Names
PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
PIKFYVE, Antibody; PIKFYVE Polyclonal Antibody; PIP5K3: CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29; anti-PIKFYVE antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
2098
Applicable Applications for anti-PIKFYVE antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 269-545 of human PIKFYVE (NP_055855.2).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of Mouse lung, using PIKFYVE antibody (AAA37642) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of Mouse lung, using PIKFYVE antibody (AAA37642) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
Related Product Information for anti-PIKFYVE antibody
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles.
Product Categories/Family for anti-PIKFYVE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 250kDa
Calculated MW: 237kDa
Calculated MW: 237kDa
NCBI Official Full Name
1-phosphatidylinositol 3-phosphate 5-kinase
NCBI Official Synonym Full Names
phosphoinositide kinase, FYVE finger containing
NCBI Official Symbol
PIKFYVE
NCBI Official Synonym Symbols
CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
NCBI Protein Information
1-phosphatidylinositol 3-phosphate 5-kinase; PIPkin-III; type III PIP kinase; epididymis luminal protein 37; zinc finger, FYVE domain containing 29; 1-phosphatidylinositol-3-phosphate 5-kinase; phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
UniProt Protein Name
1-phosphatidylinositol 3-phosphate 5-kinase
UniProt Gene Name
PIKFYVE
UniProt Synonym Gene Names
KIAA0981; PIP5K3; Phosphatidylinositol 3-phosphate 5-kinase; PIPkin-III; Type III PIP kinase
UniProt Entry Name
FYV1_HUMAN
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Product Notes
The PIKFYVE pikfyve (Catalog #AAA37642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIKFYVE Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIKFYVE can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PIKFYVE pikfyve for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIKFYVE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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