Rabbit PION Polyclonal Antibody | anti-PION antibody

PION Antibody

Cat. Num. AAA40325

• Gene Names: GSAP; PION
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: PION Antibody is affinity chromatography purified via peptide column.
• Synonyms: PION, Antibody; PION Antibody; PION; Gamma-secretase-activating protein; Protein pigeon homolog; GSAP; pigeon homolog (Drosophila); anti-PION antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoforms of PION are known to exist. PION antibody is predicted to not cross-react with other F-box protein family members.
• Purity/Purification: PION Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Positions: 770-820
• Sequence Length: 820

• Applicable Applications for anti-PION antibody: WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
• Conjugate: Unconjugated
• Immunogen: PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION.
• Buffer: PION Antibody is supplied in PBS containing 0.02% sodium azide.
• Positive control: MBS151675- EL4 Cell Lysate; MBS154323- Human Brain Tissue Slide.
• Preparation and Storage: PION antibody can be stored at 4°C for three months and -20° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 μg/mL.Red: PION Antibody (6161)Blue: DAPI staining)

IHC (Immunohistochemistry)

(Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-PION antibody

PION Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99 -amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimer's disease.

NCBI and Uniprot Product Information

• NCBI GI #: 148612805
• NCBI GeneID: 54103
• NCBI Accession #: NP_059135
• NCBI GenBank Nucleotide #: NM_017439.3
• UniProt Accession #: A4D1B5; Q3MJC0; Q8ND73; Q9UMH3; Q9Y4L9; A4D1B6
• NCBI Official Full Name: gamma-secretase-activating protein
• NCBI Official Synonym Full Names: gamma-secretase activating protein
• NCBI Official Symbol: GSAP
• NCBI Official Synonym Symbols: PION
• NCBI Protein Information: gamma-secretase-activating protein; pigeon homolog; protein pigeon homolog
• UniProt Protein Name: Gamma-secretase-activating protein
• UniProt Gene Name: GSAP
• UniProt Synonym Gene Names: PION; GSAP; GSAP-16K
• UniProt Entry Name: GSAP_HUMAN