Application Data
(Anti-Pilx2 (Human C-term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit PITX2 Polyclonal Antibody | anti-PITX2 antibody
PITX2 Antibody (C-term)
Gene Names
PITX2; RS; RGS; ARP1; Brx1; IDG2; IGDS; IHG2; PTX2; RIEG; IGDS2; IRID2; Otlx2; RIEG1
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Zebrafish, Chicken, Xenopus)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PITX2, Antibody; PITX2 Antibody (C-term); Pituitary homeobox 2; ALL1-responsive protein ARP1; Homeobox protein PITX2; Paired-like homeodomain transcription factor 2; RIEG bicoid-related homeobox transcription factor; Solurshin; PITX2; ARP1; RGS; RIEG; RIEG1; anti-PITX2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Zebrafish, Chicken, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids of human PITX2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
122-151
Sequence Length
271
Applicable Applications for anti-PITX2 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Function
Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform PTX2C is involved in left-right asymmetry the developing embryo (By similarity).
Cellular Location
Nucleus.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application Data
(Anti-Pilx2 (Human C-term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Anti-Pilx2 (Human C-term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Anti-Pilx2 (Human C-term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Anti-Pilx2 (Human C-term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with PITX2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PITX2 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with PITX2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PITX2 immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-PITX2 antibody
Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.
Product Categories/Family for anti-PITX2 antibody
References
Engenheiro,E., Clin. Genet. 72 (5), 464-470 (2007)
Gudbjartsson,D.F., Nature 448 (7151), 353-357 (2007)
Lowry,R.B., Am. J. Med. Genet. A 143 (11), 1227-1230 (2007)
Gudbjartsson,D.F., Nature 448 (7151), 353-357 (2007)
Lowry,R.B., Am. J. Med. Genet. A 143 (11), 1227-1230 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
pituitary homeobox 2 isoform c
NCBI Official Synonym Full Names
paired-like homeodomain 2
NCBI Official Symbol
PITX2
NCBI Official Synonym Symbols
RS; RGS; ARP1; Brx1; IDG2; IGDS; IHG2; PTX2; RIEG; IGDS2; IRID2; Otlx2; RIEG1
NCBI Protein Information
pituitary homeobox 2
UniProt Protein Name
Pituitary homeobox 2
UniProt Gene Name
PITX2
UniProt Synonym Gene Names
ARP1; RGS; RIEG; RIEG1
UniProt Entry Name
PITX2_HUMAN
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Product Notes
The PITX2 pitx2 (Catalog #AAA287284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 122-151. The PITX2 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat, Zebrafish, Chicken, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's PITX2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PITX2 pitx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PITX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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