WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PITX2 antibody (AAA37029) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Rabbit PITX2 Polyclonal Antibody | anti-PITX2 antibody
PITX2
Gene Names
PITX2; RS; RGS; ARP1; Brx1; IDG2; IGDS; IHG2; PTX2; RIEG; IGDS2; IRID2; Otlx2; RIEG1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
PITX2, Antibody; PITX2; ARP1;ASGD4;Brx1;IDG2;IGDS;IGDS2;IHG2;IRID2;Otlx2;PTX2;RGS;RIEG;RIEG1;RS;ARP1;Brx1;IDG2;IGDS;IGDS2;IHG2;IRID2;Otlx2;PTX2;RGS;RIEG;RIEG1;RS; anti-PITX2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence Length
317
Applicable Applications for anti-PITX2 antibody
WB (Western Blot)
Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PITX2 (NP_000316.2).
Category
Primary antibody
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PITX2 antibody (AAA37029) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PITX2 antibody (AAA37029) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Related Product Information for anti-PITX2 antibody
Background: This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Product Categories/Family for anti-PITX2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 30kDa/35kDa
Observed MW: 39kDa
Observed MW: 39kDa
NCBI Official Full Name
Pituitary homeobox 2
NCBI Official Synonym Full Names
paired-like homeodomain 2
NCBI Official Symbol
PITX2
NCBI Official Synonym Symbols
RS; RGS; ARP1; Brx1; IDG2; IGDS; IHG2; PTX2; RIEG; IGDS2; IRID2; Otlx2; RIEG1
NCBI Protein Information
pituitary homeobox 2; solurshin; all1-responsive gene 1; homeobox protein PITX2; ALL1-responsive protein ARP1; paired-like homeodomain transcription factor 2; rieg bicoid-related homeobox transcription factor 1
UniProt Protein Name
Pituitary homeobox 2
UniProt Gene Name
PITX2
UniProt Synonym Gene Names
ARP1; RGS; RIEG; RIEG1
UniProt Entry Name
PITX2_HUMAN
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Product Notes
The PITX2 pitx2 (Catalog #AAA37029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PITX2 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PITX2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PITX2 pitx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PITX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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