Rabbit anti-Human, Mouse PIWI-L2 Polyclonal Antibody | anti-PIWIL2 antibody

PIWI-L2 Antibody

Cat. Num. AAA39590

• Gene Names: PIWIL2; CT80; HILI; mili; PIWIL1L
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: PIWI-L2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PIWI-L2, Antibody; PIWI-L2 Antibody; PIWI-L2; CT80; HILI; mili; PIWIL1L; Piwi-like protein 2; piwi-like 2; anti-PIWIL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.
• Purity/Purification: PIWI-L2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 973

• Applicable Applications for anti-PIWIL2 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410-460 of PIWI-L2
• Preparation and Storage: PIWI-L2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 5 μg/ml.)

ICC (Immunocytochemistry)

(Immunocytochemistry of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 2.5 μg/ml.)

WB (Western Blot)

(Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL.)

Related Product Information for anti-PIWIL2 antibody

PIWI-L2 Antibody: PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 24431985
• NCBI GeneID: 55124
• NCBI Accession #: NP_00129193
• NCBI GenBank Nucleotide #: NM_018068.3
• UniProt Accession #: Q8TC59; Q96SW6; Q9NW28; A8K4S3; A8K8S5; B0AZN9; B0AZP2; B4DR22; E7ECA4
• Molecular Weight: 105,760 Da
• NCBI Official Full Name: piwi-like protein 2
• NCBI Official Synonym Full Names: piwi-like RNA-mediated gene silencing 2
• NCBI Official Symbol: PIWIL2
• NCBI Official Synonym Symbols: CT80; HILI; mili; PIWIL1L
• NCBI Protein Information: piwi-like protein 2; Miwi like; piwi-like 2; piwil2-like protein; cancer/testis antigen 80; 80kDa PIWIL2 short isoform
• UniProt Protein Name: Piwi-like protein 2
• UniProt Gene Name: PIWIL2
• UniProt Synonym Gene Names: HILI; CT80
• UniProt Entry Name: PIWL2_HUMAN