Rabbit PKM2 Polyclonal Antibody | anti-PKM antibody

PKM2 (N-term E131) Antibody

Cat. Num. AAA289922

• Gene Names: PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Reactivity: Human (Predicted Reactivity: Mouse, Rabbit, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Synonyms: PKM2, Antibody; PKM2 (N-term E131) Antibody; Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58; PKM; OIP3; PK2; PK3; PKM2; anti-PKM antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rabbit, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the N-terminal region of human PKM2.
• Purity/Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 116-145
• Sequence Length: 516

• Applicable Applications for anti-PKM antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed:15996096, PubMed:1854723, PubMed:20847263). The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed:15996096, PubMed:1854723, PubMed:20847263). The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival (PubMed:15996096, PubMed:1854723, PubMed:20847263).
• Cellular Location: [Isoform M2]: Cytoplasm. Nucleus Note=Translocates to the nucleus in response to various signals, such as EGF receptor activation or apoptotic stimuli (PubMed:17308100, PubMed:22056988, PubMed:24120661). Nuclear translocation is promoted by acetylation by EP300 (PubMed:24120661). Deacetylation by SIRT6 promotes its nuclear export in a process dependent of XPO4, thereby suppressing its ability to activate transcription and promote tumorigenesis (PubMed:26787900).
• Tissue Location: [Isoform M2]: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

FCM/FACS (Flow Cytometry)

(PKM2 (N-term E131) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(PKM2 (N-term E131) Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 (N-term E131) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of PKM2 (arrow) using rabbit polyclonal PKM2 Antibody (N-term E131). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).)

Related Product Information for anti-PKM antibody

PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Product Categories/Family for anti-PKM antibody

Cancer; Cardiovascular; Metabolism; Signal transduction

References

Clower,C.V., et.al., Proc. Natl. Acad. Sci. U.S.A. 107 (5), 1894-1899 (2010)
David,C.J., et.al., Nature 463 (7279), 364-368 (2010)

NCBI and Uniprot Product Information

• NCBI GI #: 332164775
• NCBI GeneID: 5315
• NCBI Accession #: NP_001193725.1; NP_001193726.1; NP_001193727.1; NP_001193728.1; NP_002645.3; NP_872270.1; NP_872271.1
• NCBI GenBank Nucleotide #: NM_001206796.1
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
• Molecular Weight: 57937
• NCBI Official Full Name: pyruvate kinase PKM isoform c
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN