Application Data
Rabbit PLCg1 (Tyr-775) Polyclonal Antibody | anti-PLCG1 antibody
Anti-PLC-gamma1 (Tyr-775), phospho-specific
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Rat, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Western Blot, ELISA
Purity
Affinity Purified
Synonyms
PLCg1 (Tyr-775), Antibody; Anti-PLC-gamma1 (Tyr-775), phospho-specific; PLCg1 (Tyr-775); phospho-specific; Phospholipase C gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; anti-PLCG1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-PLC-gamma-1 (Tyr-775) peptide. The antibody detects a 150 kDa* protein in human Jurkat and A431 cells treated with pervanadate, but is not observed in untreated cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Sequence Length
1291
Applicable Applications for anti-PLCG1 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
PLC-gamma-1 (Tyr-775) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 775 in human PLC-gamma-1. This sequence has high homology to the conserved site in rat and mouse PLC-gamma-1, and has low homology to PLC-gamma-2.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Application Data
Application Data
Related Product Information for anti-PLCG1 antibody
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLC-beta, PLC-gamma, PLC-delta, and PLC-epsilon. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLC-delta is activated by both receptor and nonreceptor tyrosine kinases. PLC-gamma-1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLC-gamma-1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity.
References
• Obermeier, A. et al. (1996) EMBO J. 15:73.
• Chen, P. et al. (1996) J Cell. Biol. 134:689.
• Serrano, C.J. et al. (2005) J. Immunol. 174:6233.
• Chen, P. et al. (1996) J Cell. Biol. 134:689.
• Serrano, C.J. et al. (2005) J. Immunol. 174:6233.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
148530 Da
NCBI Official Full Name
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 isoform a
NCBI Official Synonym Full Names
phospholipase C, gamma 1
NCBI Official Symbol
PLCG1
NCBI Official Synonym Symbols
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
NCBI Protein Information
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; OTTHUMP00000031787; OTTHUMP00000178982; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylinositol phospholipase C; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-gamma-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; phospholipase C, gamma 1 (formerly subtype 148); 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
UniProt Protein Name
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
UniProt Gene Name
PLCG1
UniProt Synonym Gene Names
PLC1
UniProt Entry Name
PLCG1_HUMAN
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Product Notes
The PLCG1 plcg1 (Catalog #AAA71528) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLC-gamma1 (Tyr-775), phospho-specific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLCg1 (Tyr-775) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PLCG1 plcg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCg1 (Tyr-775), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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