WB (Western Blot)
(Western blot analysis of extracts of Ramos cells, using Phospho-PLCgamma2 (Tyr1217) Antibody.)
Rabbit PLCgamma2 Polyclonal Antibody | anti-PLCG2 antibody
Phospho-PLCgamma2 (Tyr1217) Antibody
Gene Names
PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
PLCgamma2, Antibody; Phospho-PLCgamma2 (Tyr1217) Antibody; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase gamma-2; EC 3.1.4.11; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-2; Phospholipase C gamma 2; Phospholipase C, gamma 2 (phosphatidylinositol specific); Phospholipase C-gamma-2; Phospholipase C-IV; PLC 2; PLC gamma 2; PLC IV; PLC-gamma-2; PLC-IV; Plcg2; PLCG2_HUMAN; anti-PLCG2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-PLCgamma2 (Tyr1217) antibody detects endogenous levels of PLCgamma2.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1265
Applicable Applications for anti-PLCG2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PLCgamma2 around the phosphorylation site of Tyr1217.
Subcellular Location
Cytoplasmic and Plasma Membrane
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (83%)
Conjugation
Unconjugated
Epitope
Phospho Tyr1217
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of Ramos cells, using Phospho-PLCgamma2 (Tyr1217) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of Ramos cells, using Phospho-PLCgamma2 (Tyr1217) Antibody.)
IHC (Immunohistochemistry)
(AAA321368 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321368 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PLCG2 antibody
Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Subunit Structure: Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).
Post-translational Modifications: Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity.
Subunit Structure: Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).
Post-translational Modifications: Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 150 kDa
Predicted: 148 kDa
Predicted: 148 kDa
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
NCBI Official Synonym Full Names
phospholipase C gamma 2
NCBI Official Symbol
PLCG2
NCBI Official Synonym Symbols
FCAS3; APLAID; PLC-IV; PLC-gamma-2
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
UniProt Gene Name
PLCG2
UniProt Synonym Gene Names
PLC-IV; PLC-gamma-2
Similar Products
Product Notes
The PLCG2 plcg2 (Catalog #AAA321368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PLCgamma2 (Tyr1217) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLCgamma2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLCG2 plcg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCgamma2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.