WB (Western Blot)
(Detection of human PLCG2 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, RKO, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLCG2 antibody (AAA213481 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human PLCG2 Polyclonal Antibody | anti-PLCG2 antibody
Rabbit anti-PLCG2 Antibody, Affinity Purified
Gene Names
PLCG2; FCAS3; APLAID
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PLCG2, Antibody; Rabbit anti-PLCG2 Antibody, Affinity Purified; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; Phospholipase C-gamma-2; PLC-IV; PLC-gamma-2; phospholipase C-IV; phospholipase C, gamma 2 (phosphatidylinositol-specific); phosphoinositide phospholipase C-gamma-2; APLAID; FCAS3; phospholipase C gamma 2; anti-PLCG2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PLCG2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 500 and 550
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PLCG2 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, RKO, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLCG2 antibody (AAA213481 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PLCG2 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, RKO, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLCG2 antibody (AAA213481 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PLCG2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLCG2 antibody (AAA213481 lot 1) used for IP at 1 ug per reaction. PLCG2 was also immunoprecipitated by rabbit anti-PLCG2 antibody BL24623. For blotting immunoprecipitated PLCG2, AAA213481 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PLCG2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLCG2 antibody (AAA213481 lot 1) used for IP at 1 ug per reaction. PLCG2 was also immunoprecipitated by rabbit anti-PLCG2 antibody BL24623. For blotting immunoprecipitated PLCG2, AAA213481 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PLCG2 antibody
Activation of PLCG2 results in the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It is a crucial enzyme in transmembrane signaling. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P16885].
Product Categories/Family for anti-PLCG2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147,870 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
NCBI Official Synonym Full Names
phospholipase C, gamma 2 (phosphatidylinositol-specific)
NCBI Official Symbol
PLCG2
NCBI Official Synonym Symbols
FCAS3; APLAID
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; PLC-IV; PLC-gamma-2; phospholipase C-IV; phosphoinositide phospholipase C-gamma-2
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
UniProt Gene Name
PLCG2
UniProt Synonym Gene Names
PLC-IV; PLC-gamma-2
UniProt Entry Name
PLCG2_HUMAN
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Product Notes
The PLCG2 plcg2 (Catalog #AAA213481) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PLCG2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLCG2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PLCG2 plcg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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