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product-image-AAA288656_FCM11.jpg FCM/FACS (Flow Cytometry) (PLG Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Human PLG Polyclonal Antibody | anti-PLG antibody

PLG Antibody (C-term)

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Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PLG, Antibody; PLG Antibody (C-term); Plasminogen; Plasmin heavy chain A; Activation peptide; Angiostatin; short form; Plasmin light chain B; PLG; anti-PLG antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-665 amino acids from the C-terminal region of human PLG.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
636-665
Sequence Length
810
Applicable Applications for anti-PLG antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(PLG Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA288656_FCM11.jpg FCM/FACS (Flow Cytometry) (PLG Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PLG Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

product-image-AAA288656_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PLG Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of PLG antibody (C-term) in K562 cell line lysates (35ug/lane). PLG (arrow) was detected using the purified Pab.)

product-image-AAA288656_WB15.jpg WB (Western Blot) (Western blot analysis of PLG antibody (C-term) in K562 cell line lysates (35ug/lane). PLG (arrow) was detected using the purified Pab.)
Related Product Information for anti-PLG antibody
PLG is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase and tissue plasminogen activator. The main function of this protein is to dissolve fibrin clots. The protein, like trypsin, belongs to the family of serine proteinases.
Product Categories/Family for anti-PLG antibody
References
Hofmann,S.C., Voith,U. J. Invest. Dermatol. 129 (7), 1730-1739 (2009)
Passero,C.J., Mueller,G.M. J. Biol. Chem. 283 (52), 36586-36591 (2008)
Ohyama,S., Harada,T. Eur. J. Biochem. 271 (4), 809-820 (2004)
Lee,H., Kim,H.K. Arch. Biochem. Biophys. 375 (2), 359-363 (2000)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90569
NCBI Official Full Name
plasminogen isoform 1
NCBI Official Synonym Full Names
plasminogen
NCBI Official Symbol
PLG
NCBI Protein Information
plasminogen
UniProt Protein Name
Plasminogen
UniProt Gene Name
PLG
UniProt Entry Name
PLMN_HUMAN

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Product Notes

The PLG plg (Catalog #AAA288656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 636-665. The PLG Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLG can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PLG plg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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