Rabbit PLK1 / PLK-1 Polyclonal Antibody | anti-PLK1 antibody

Rabbit Polyclonal to Human PLK1 / PLK-1

Cat. Num. AAA52108

• Gene Names: PLK1; PLK; STPK13
• Reactivity: Bovine, Dog, Human, Macaque, Monkey, Mouse, Pig, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antiserum
• Synonyms: PLK1 / PLK-1, Antibody; Rabbit Polyclonal to Human PLK1 / PLK-1; Anti-PLK1 / PLK-1 Antibody (aa100-200) IHC-plus; PLK1; Polo like kinase; Polo-like kinase (Drosophila); Polo-like kinase 1; STPK13; Polo (Drosophia)-like kinase; PLK; PLK-1; Human PLK1; anti-PLK1 antibody

• Host: Rabbit
• Reactivity: Bovine, Dog, Human, Macaque, Monkey, Mouse, Pig, Rat
• Clonality: Polyclonal
• Specificity: A portion of amino acids 100-200 of human PLK1 was used as the immunogen.
• Purity/Purification: Antiserum
• Form/Format: PBS containing 0.05% BSA and 0.05% sodium azide
• Sequence Length: 603

• Applicable Applications for anti-PLK1 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Family/Subfamily: Protein Kinase/Polo
• Antigen type: Protein fragment
• Immunogen: PLK1/PLK-1 antibody was raised against a portion of amino avids 100-200 of human PLK1.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

WB (Western Blot)

(Western blot of PLK1 in HCT-116 using antibody at 1:2000.)

IHC (Immunohistochemistry)

(Anti-PLK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Related Product Information for anti-PLK1 antibody

PLK, a Polo type protein kinase, has been shown to function in mitosis. The mRNA is absent or expressed at very low levels during the G1 phase, begins to reaccumulate during the S phase, and reaches maximal levels during the G2/M phase. PLK1 may participate in targeting MPF to the nucleus during prophase and in regulating mitotic spindle function. These functions, in addition to its expression in proliferating cells, suggest that PLK1 has a role in cancer progression.

Product Categories/Family for anti-PLK1 antibody

Family: Protein Kinase; Subfamily: Polo

NCBI and Uniprot Product Information

• NCBI GI #: 21359873
• NCBI GeneID: 5347
• NCBI Accession #: NP_005021.2
• NCBI GenBank Nucleotide #: NM_005030.3
• UniProt Accession #: P53350; Q15153; Q99746
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo-like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1; PLK-1; polo like kinase; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; cell cycle regulated protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13
• UniProt Entry Name: PLK1_HUMAN