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WB (Western Blot)
(Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.)
Rabbit anti-Human, mouse PMCH Polyclonal Antibody | anti-PMCH antibody
PMCH Antibody (Center)
Gene Names
PMCH; MCH
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PMCH, Antibody; PMCH Antibody (Center); Pro-MCH; Neuropeptide-glycine-glutamic acid; NGE; Neuropeptide G-E; Neuropeptide-glutamic acid-isoleucine; NEI; Neuropeptide E-I; Melanin-concentrating hormone; MCH; PMCH; anti-PMCH antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PMCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the Central region of human PMCH.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
94-122
Sequence Length
165
Applicable Applications for anti-PMCH antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.)
WB (Western Blot)
(Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.)
FCM/FACS (Flow Cytometry)
(PMCH Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PMCH Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(PMCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(PMCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PMCH Antibody (Center) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).)
WB (Western Blot)
(PMCH Antibody (Center) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).)
WB (Western Blot)
(PMCH Antibody (Center) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).)
WB (Western Blot)
(PMCH Antibody (Center) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).)
Related Product Information for anti-PMCH antibody
The melanin-concentrating hormone (MCH) is a cyclic
neuropeptide isolated initially from salmon pituitary gland and
later from rat hypothalamus (summarized by Nahon et al., 1992
[PubMed 1572663]). In mammals, MCH perikarya are confined largely
to the lateral hypothalamus and zona incerta area with extensive
neuronal projections throughout the brain, including the
neurohypophysis. The anatomic distribution suggests a
neurotransmitter or neuromodulator role for MCH in a broad array of
neuronal functions directed toward the regulation of goal-directed
behavior, such as food intake, and general arousal. MCH and 2 other
putative neuropeptides, NEI and NGE, are encoded by the same
precursor and appear colocalized in nerve cells and in many
instances within the projections. The precursor is designated
pro-melanin-concentrating hormone (PMCH).
neuropeptide isolated initially from salmon pituitary gland and
later from rat hypothalamus (summarized by Nahon et al., 1992
[PubMed 1572663]). In mammals, MCH perikarya are confined largely
to the lateral hypothalamus and zona incerta area with extensive
neuronal projections throughout the brain, including the
neurohypophysis. The anatomic distribution suggests a
neurotransmitter or neuromodulator role for MCH in a broad array of
neuronal functions directed toward the regulation of goal-directed
behavior, such as food intake, and general arousal. MCH and 2 other
putative neuropeptides, NEI and NGE, are encoded by the same
precursor and appear colocalized in nerve cells and in many
instances within the projections. The precursor is designated
pro-melanin-concentrating hormone (PMCH).
Product Categories/Family for anti-PMCH antibody
References
Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009) :
Aziz, A., et al. Brain Pathol. 18(4):474-483(2008)
Kokkotou, E., et al. Proc. Natl. Acad. Sci. U.S.A. 105(30):10613-10618(2008)
Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009) :
Aziz, A., et al. Brain Pathol. 18(4):474-483(2008)
Kokkotou, E., et al. Proc. Natl. Acad. Sci. U.S.A. 105(30):10613-10618(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18679
NCBI Official Full Name
pro-MCH preproprotein
NCBI Official Synonym Full Names
pro-melanin-concentrating hormone
NCBI Official Symbol
PMCH
NCBI Official Synonym Symbols
MCH
NCBI Protein Information
pro-MCH
UniProt Protein Name
Pro-MCH
UniProt Gene Name
PMCH
UniProt Synonym Gene Names
MCH; NGE; Neuropeptide G-E; NEI; Neuropeptide E-I; MCH
UniProt Entry Name
MCH_HUMAN
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Product Notes
The PMCH pmch (Catalog #AAA289305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 94-122. The PMCH Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PMCH can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PMCH pmch for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMCH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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