IHC (Immunohiostchemistry)
(Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.)
Rabbit PMEPA1 Polyclonal Antibody | anti-PMEPA1 antibody
PMEPA1 Antibody
Gene Names
PMEPA1; STAG1; TMEPAI
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
PMEPA1, Antibody; PMEPA1 Antibody; Prostate transmembrane protein androgen induced 1, STAG1, TMEPAI; anti-PMEPA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
287
Applicable Applications for anti-PMEPA1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
A549 Cell Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
IHC (Immunohiostchemistry)
(Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.)
WB (Western Blot)
(Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot)
(Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-PMEPA1 antibody
The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).
Product Categories/Family for anti-PMEPA1 antibody
References
Xu LL, Shanmugam N, Segawa T, et al. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q!3 exhibits high level expression in prostate. Genomics 2000; 66:257-63. Rae FK, Hooper JD, Nicol DL, et al. Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. Mol. Carcinog. 2001; 32:44-53. Watanabe Y, Itoh S, Goto T, et al. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol. Cell 2010; 37:123-34. Vo Nguyen TT, Watanabe Y, Shiba A, et al. TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells. Cancer Sci. 2014; 105:334-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 26, 28, 32 kDa
Observed: 27 kDa
Observed: 27 kDa
NCBI Official Full Name
protein TMEPAI isoform a
NCBI Official Synonym Full Names
prostate transmembrane protein, androgen induced 1
NCBI Official Symbol
PMEPA1
NCBI Official Synonym Symbols
STAG1; TMEPAI
NCBI Protein Information
protein TMEPAI
UniProt Protein Name
Protein TMEPAI
UniProt Gene Name
PMEPA1
UniProt Synonym Gene Names
STAG1; TMEPAI
UniProt Entry Name
PMEPA_HUMAN
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Product Notes
The PMEPA1 pmepa1 (Catalog #AAA40854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PMEPA1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PMEPA1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PMEPA1 pmepa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMEPA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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