WB (Western Blot)
(Detection of human PML by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, GaMG, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PML antibody AAA211341 (AAA211341 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PML Polyclonal Antibody | anti-PML antibody
Rabbit anti-PML Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PML, Antibody; Rabbit anti-PML Antibody, Affinity Purified; RNF71; protein PML; TRIM19; tripartite motif-containing protein 19; RING finger protein 71; promyelocytic leukemia protein; probable transcription factor PML; PP8675; PML/RARA fusion; MYL; tripartite motif protein TRIM19; promyelocytic leukemia, inducer of; Protein PML; promyelocytic leukemia; anti-PML antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PML antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PML by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, GaMG, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PML antibody AAA211341 (AAA211341 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PML by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, GaMG, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PML antibody AAA211341 (AAA211341 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PML by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PML antibody (AAA211341 lot 4) used for IP at 6 ug per reaction. PML was also immunoprecipitated by a previous lot of this antibody (AAA211341 lot 3) and a second antibody against a different epitope of PML . For blotting immunoprecipitated PML, AAA211341 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human PML by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PML antibody (AAA211341 lot 4) used for IP at 6 ug per reaction. PML was also immunoprecipitated by a previous lot of this antibody (AAA211341 lot 3) and a second antibody against a different epitope of PML . For blotting immunoprecipitated PML, AAA211341 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human PML by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PML (Cat. No. AAA211341 Lot4) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PML by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PML (Cat. No. AAA211341 Lot4) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-PML antibody
PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.
Product Categories/Family for anti-PML antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PML pml (Catalog #AAA211341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PML Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PML can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PML pml for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PML, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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