Rabbit anti-Human Podoplanin Polyclonal Antibody | anti-PDPN antibody

Anti-Podoplanin Antibody, Rabbit Polyclonal

Cat. Num. AAA255312

• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Podoplanin, Antibody; Anti-Podoplanin Antibody, Rabbit Polyclonal; Podoplanin/PDPN Antibody, Rabbit PAb, Antigen Affinity Purified; podoplanin; Anti-AGGRUS Antibody; Anti-GP36 Antibody; Anti-Gp38 Antibody; Anti-GP40 Antibody; Anti-HT1A-1 Antibody; Anti-OTS8 Antibody; Anti-PA2.26 Antibody; Anti-T1A Antibody; Anti-T1A-2 Antibody; Anti-T1A2 Antibody; Anti-TI1A Antibody; anti-PDPN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Podoplanin/PDPN
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PDPN antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human Podoplanin/PDPN Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Podoplanin/PDPN (rh Podoplanin/PDPN; Q86YL7-1; Met1-Lys123). Podoplanin/PDPN specific IgG was purified by Human Podoplanin/PDPN affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(PDPN was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate1 uL anti-PDPN rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PDPN rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 41 kDa)

WB (Western Blot)

(Anti-PDPN rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:41 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-PDPN antibody

Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a marker for epithelioid hemangioendothelioma in liver tumors.

References

Kimura, N. et al., 2005, Pathol Int. 55 (2): 83-86. Ordonez, N.G., 2006, Adv Anat Pathol. 13 (2): 83-88. Wicki, A. et al., 2007, Br. J. Cancer. 96 (1): 1-5. Fujii, T. et al., 2008, Mod Pathol. 21 (2): 125-130. Sawa, Y. et al., 2008, Acta Histochem Cytochem. 41 (5):121-126. Kaddu, S. et al., 2009, Am J Dermatopathol. 31 (2): 137-139.