Rabbit anti-Human, Mouse POLD3 Polyclonal Antibody | anti-POLD3 antibody

POLD3 Antibody

Cat. Num. AAA234135

• Gene Names: POLD3; P66; P68; PPP1R128
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: POLD3, Antibody; POLD3 Antibody; DNA polymerase delta subunit 3; DNA polymerase delta subunit C1; POLD3; KIAA0039; anti-POLD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 466

• Applicable Applications for anti-POLD3 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human DNA polymerase delta subunit 3 protein (203-275AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohiostchemistry)

(IHC image of AAA234135 diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in: Mouse liver tissueAll lanes: POLD3 antibody at 5.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52, 47, 40 KDaObserved band size: 52 KDa)

Related Product Information for anti-POLD3 antibody

As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).

Product Categories/Family for anti-POLD3 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 38492356
• NCBI GeneID: 10714
• NCBI Accession #: NP_006582.1
• NCBI GenBank Nucleotide #: NM_006591.2
• UniProt Accession #: Q15054; Q32MZ9; Q32N00; B7ZAI6
• Molecular Weight: 39,320 Da
• NCBI Official Full Name: DNA polymerase delta subunit 3
• NCBI Official Synonym Full Names: DNA polymerase delta 3, accessory subunit
• NCBI Official Symbol: POLD3
• NCBI Official Synonym Symbols: P66; P68; PPP1R128
• NCBI Protein Information: DNA polymerase delta subunit 3
• UniProt Protein Name: DNA polymerase delta subunit 3
• UniProt Gene Name: POLD3
• UniProt Synonym Gene Names: KIAA0039