WB (Western Blot)
(Detection of human PolD3/p66 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolD3/p66 antibody AAA211376 (lot AAA211376-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PolD3/p66 Polyclonal Antibody | anti-PolD3/p66 antibody
Rabbit anti-PolD3/p66 Antibody, Affinity Purified
Gene Names
POLD3; P66; P68
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PolD3/p66, Antibody; Rabbit anti-PolD3/p66 Antibody, Affinity Purified; PPP1R128; protein phosphatase 1, regulatory subunit 128; polymerase (DNA-directed), delta 3, accessory subunit; polymerase (DNA) delta 3, accessory subunit; Pol delta C subunit (p66); P66; DNA polymerase delta subunit p68; DNA polymerase delta subunit p66; DNA polymerase delta subunit C; DNA polymerase delta subunit 3; P68; DNA polymerase delta 3, accessory subunit; anti-PolD3/p66 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PolD3/p66 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 416 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PolD3/p66 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolD3/p66 antibody AAA211376 (lot AAA211376-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PolD3/p66 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolD3/p66 antibody AAA211376 (lot AAA211376-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PolD3/p66 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolD3/p66 antibody AAA211376 (lot AAA211376-2) used for IP at 6 ug per reaction. PolD3/p66 was also immunoprecipitated by rabbit anti-PolD3/p66 antibody For blotting immunoprecipitated PolD3/p66, AAA211376 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PolD3/p66 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolD3/p66 antibody AAA211376 (lot AAA211376-2) used for IP at 6 ug per reaction. PolD3/p66 was also immunoprecipitated by rabbit anti-PolD3/p66 antibody For blotting immunoprecipitated PolD3/p66, AAA211376 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PolD3/p66 antibody
PolD3/p66 is a subunit of the heterotetrameric DNA polymerase delta that plays a role in DNA replication and repair. The PolD3/p66 subunit has been shown to mediate the interaction between DNA polymerase delta and PCNA (proliferating cell nuclear antigen) to facilitate processivity.
Product Categories/Family for anti-PolD3/p66 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,400 Da
NCBI Official Full Name
DNA polymerase delta subunit 3
NCBI Official Synonym Full Names
polymerase (DNA-directed), delta 3, accessory subunit
NCBI Official Symbol
POLD3
NCBI Official Synonym Symbols
P66; P68
NCBI Protein Information
DNA polymerase delta subunit 3; Pol delta C subunit (p66); DNA polymerase delta subunit p66
UniProt Protein Name
DNA polymerase delta subunit 3
UniProt Gene Name
POLD3
UniProt Synonym Gene Names
KIAA0039
UniProt Entry Name
DPOD3_HUMAN
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Product Notes
The PolD3/p66 pold3 (Catalog #AAA211376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PolD3/p66 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PolD3/p66 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PolD3/p66 pold3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PolD3/p66, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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