FCM/FACS (Flow Cytometry)
(POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit POLR1B Polyclonal Antibody | anti-POLR1B antibody
POLR1B Antibody (N-term)
Gene Names
POLR1B; RPA2; RPA135; Rpo1-2
Reactivity
Human (Predicted Reactivity: Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POLR1B, Antibody; POLR1B Antibody (N-term); DNA-directed RNA polymerase I subunit RPA2; RNA polymerase I subunit 2; DNA-directed RNA polymerase I 135 kDa polypeptide; RPA135; POLR1B; anti-POLR1B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
89-117
Sequence Length
1079
Applicable Applications for anti-POLR1B antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(POLR1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(POLR1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(POLR1B Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).)
WB (Western Blot)
(POLR1B Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).)
Related Product Information for anti-POLR1B antibody
Eukaryotic RNA polymerase I (pol I) is responsible for the
transcription of ribosomal RNA (rRNA) genes and production of rRNA,
the primary component of ribosomes. Pol I is a multisubunit enzyme
composed of 6 to 14 polypeptides, depending on the species. Most of
the mass of the pol I complex derives from the 2 largest subunits,
Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and
Grummt, 1996 [PubMed 8921381]).
transcription of ribosomal RNA (rRNA) genes and production of rRNA,
the primary component of ribosomes. Pol I is a multisubunit enzyme
composed of 6 to 14 polypeptides, depending on the species. Most of
the mass of the pol I complex derives from the 2 largest subunits,
Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and
Grummt, 1996 [PubMed 8921381]).
References
Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128229
NCBI Official Full Name
DNA-directed RNA polymerase I subunit RPA2 isoform 2
NCBI Official Synonym Full Names
polymerase (RNA) I polypeptide B, 128kDa
NCBI Official Symbol
POLR1B
NCBI Official Synonym Symbols
RPA2; RPA135; Rpo1-2
NCBI Protein Information
DNA-directed RNA polymerase I subunit RPA2
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA2
UniProt Gene Name
POLR1B
UniProt Synonym Gene Names
RNA polymerase I subunit 2; RPA135
UniProt Entry Name
RPA2_HUMAN
Similar Products
Product Notes
The POLR1B polr1b (Catalog #AAA283618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 89-117. The POLR1B Antibody (N-term) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's POLR1B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the POLR1B polr1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.