IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B antibody at 1: 200 dilution.)
Rabbit anti-Human POLR2B Polyclonal Antibody | anti-POLR2B antibody
POLR2B antibody
Gene Names
POLR2B; RPB2; POL2RB; hRPB140
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by antigen-affinity chromatography.
Synonyms
POLR2B, Antibody; POLR2B antibody; anti-POLR2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Sequence Length
1174
Applicable Applications for anti-POLR2B antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 142 of POLR2B
Target Name
POLR2B
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B antibody at 1: 200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B antibody at 1: 200 dilution.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using POLR2B antibody at 1: 500 dilution.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using POLR2B antibody at 1: 500 dilution.)
Application Data
(Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE Primary antibody diluted at 1: 1000)
Application Data
(Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE Primary antibody diluted at 1: 1000)
Related Product Information for anti-POLR2B antibody
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq]
Product Categories/Family for anti-POLR2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,897 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB2 isoform 1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
NCBI Official Symbol
POLR2B
NCBI Official Synonym Symbols
RPB2; POL2RB; hRPB140
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB2
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB2
UniProt Gene Name
POLR2B
UniProt Entry Name
RPB2_HUMAN
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Product Notes
The POLR2B polr2b (Catalog #AAA306848) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLR2B antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2B can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the POLR2B polr2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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