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IHC (Immunohistochemistry)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using POLR2B antibody at a dilution of 1:500)
Rabbit POLR2B Polyclonal Antibody | anti-POLR2B antibody
POLR2B Antibody
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
POLR2B, Antibody; POLR2B Antibody; Affinity Purified Rabbit Polyclonal POLR2B Antibody; Polyclonal POLR2B antibody; Anti-POLR2B antibody; hRPB140 antibody; POLRB-2 antibody; POLRB-2; hsRPB2 antibody; DNA-directed RNA polymerase II 140 kDa polypeptide antibody; POLRB 2; RPB2 antibody; POL2RB antibody; POLRB 2 antibody; POLR2B; POLR2B antibody; DNA-directed RNA polymerase II subunit B antibody; anti-POLR2B antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.65 mg/ml (varies by lot)
Sequence Length
1085
Applicable Applications for anti-POLR2B antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
POLR2B antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 172 of RNA polymerase IIB
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using POLR2B antibody at a dilution of 1:500)
IHC (Immunohistochemistry)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using POLR2B antibody at a dilution of 1:500)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B antibody at 1:200 dilution.)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 5% SDS PAGE gel and POLR2B antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 5% SDS PAGE gel and POLR2B antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: BCL-1) using a 5% SDS PAGE gel and POLR2B antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: BCL-1) using a 5% SDS PAGE gel and POLR2B antibody at a dilution of 1:1000)
Related Product Information for anti-POLR2B antibody
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Product Categories/Family for anti-POLR2B antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The POLR2B (Catalog #AAA225161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's POLR2B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the POLR2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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